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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

DNA methylation levels of SOCS1 and LINE-1 in oral epithelial cells from aggressive periodontitis patients

Texto completo
Baptista, N. B. [1] ; Portinho, D. [1] ; Casarin, R. C. V. [2] ; Vale, H. F. [2] ; Casati, M. Z. [2] ; De Souza, A. P. [1] ; Andia, D. C. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Sch Dent Piracicaba, Dept Morphol, Div Histol, Sao Paulo - Brazil
[2] Univ Estadual Campinas, Sch Dent Piracicaba, Dept Prosthodont & Periodont, Div Periodont, Piracicaba, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: ARCHIVES OF ORAL BIOLOGY; v. 59, n. 7, p. 670-678, JUL 2014.
Citações Web of Science: 6

Objective: DNA methylation has been shown to be critical in the regulation of inflammatory genes. Infections are able to trigger susceptibility to disease and it can be considered as potential epimutagenic factors in reshaping the epigenome. Therefore, what would be the DNA methylation status in cells present in an infected and inflamed oral environment? The aim was to verify the DNA methylation pattern in oral epithelium cells from aggressive periodontitis (AgP) patients in a specific gene involved in the inflammation control, as suppressor of cytokine signalling (SOCS)1 and in a broader way through long interspersed nuclear element (LINE)-1. Design: Genomic DNA from oral cells of 30 generalized AgP patients and 30 healthy patients were purified and modified by sodium bisulfite. DNA methylation patterns were analyzed using combined bisulfite restriction analysis (COBRA) for SOCS1 and LINE-1. Results: An overall scenario of demethylation was seen for both groups, whereas the healthy group presented a higher percentage of demethylation (p < 0.001), also presenting the majority of total demethylated samples (83.3% versus 70.8% in the AgP group). Total LINE-1 methylation or at each specific loci presented significant differences amongst groups. Conclusion: Epithelial cells, present in an infected and inflamed oral environment, show different DNA methylation status from those present in a healthy oral environment, regarding the SOCS1 and LINE-1. In addition, the investigation allows detecting alterations in the DNA in a non-limited manner, since the results observed might reflect a generalized condition of the oral epithelial cells, besides reflecting the condition of the gingival epithelium cells. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 10/02338-0 - Análise do padrão de metilação dos genes SOCS1, SOCS2, SOCS3 em células do epitélio gengival e em células do tecido conjuntivo gengival durante a inflamação periodontal crônica
Beneficiário:Denise Carleto Andia
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 11/07427-4 - Análise do padrão de metilação do DNA no gene SOCS1 em pacientes com periodontite agressiva
Beneficiário:Natalia Buzoli Baptista
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica