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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Enzymatic transesterification of soybean oil with ethanol using lipases immobilized on highly crystalline PVA microspheres

Texto completo
Autor(es):
Bergamasco, Juliana [1] ; de Araujo, Marcelo V. [1] ; de Vasconcellos, Adriano [1] ; Luizon Filho, Roberto A. [1] ; Hatanaka, Rafael R. [2] ; Giotto, Marcus V. [3] ; Aranda, Donato A. G. [4] ; Nery, Jose G. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, UNESP, Inst Biociencias, Dept Fis, BR-15054000 Sao Paulo - Brazil
[2] Univ Estadual Paulista, Inst Quim Araraquara, Dept Quim Organ, BR-14800900 Araraquara, SP - Brazil
[3] Univ Connecticut, Inst Mat Sci, Storrs, CT 06269 - USA
[4] Univ Fed Rio de Janeiro, Escola Quil, Lab Greentec, BR-21941972 Rio de Janeiro - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: BIOMASS & BIOENERGY; v. 59, p. 218-233, DEC 2013.
Citações Web of Science: 11
Resumo

Polyvinyl alcohol (PVA) microspheres with different degree of crystallinity were used as solid supports for Rhizomucor miehei lipase immobilization, and the enzyme-PVA complexes were used as biocatalysts for the transesterification of soybean oil to fatty acid ethyl esters (FAEE). The amounts of immobilized enzyme on the polymeric supports were similar for both the amorphous microspheres (PVA4) and the high crystalline microspheres (PVA25). However, the enzymatic activity of the immobilized enzymes was depended on the crystallinity degree of the PVA microspheres: enzymes immobilized on the PVA4 microspheres have shown low enzymatic activity (6.13 U mg(-1)), in comparison with enzymes immobilized on the high crystalline PVA25 microspheres (149.15 U mg(-1)). A synergistic effect was observed for the enzyme-PVA25 complex during the transesterification reaction of soybean oil to FAEE: transesterification reactions with free enzyme with the equivalent amount of enzyme that were immobilized onto the PVA25 microspheres (5.4 U) have yielded only 20% of FAEE, reactions with the pure highly crystalline microsphere PVA25 have not yielded FAEE, however reactions with the enzyme-PVA25 complexes have yielded 66.3% of FAEE. This synergistic effect of an immobilized enzyme on a polymeric support has not been observed before for transesterification reaction of triacylglycerides into FAEE. Based on ATR-FTIR, Na-23- and C-13-NMR-MAS spectroscopic data and the interaction of the polymeric network intermolecular hydrogen bonds with the lipases residual amino acids a possible explanation for this synergistic effect is provided. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 11/51851-5 - Síntese e caracterização de catalisadores heterogêneos a base de complexos nanozeolitas/enzimas e sua aplicação na produção de biodiesel via Rota Etílica usando como matérias-primas óleo de palma
Beneficiário:Jose Geraldo Nery
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 05/54703-6 - Síntese de catalisadores sólidos enantioseletivos: o desafio intelectual, tecnológico e sintético de zeólitas quirais
Beneficiário:Jose Geraldo Nery
Linha de fomento: Auxílio à Pesquisa - Jovens Pesquisadores
Processo FAPESP: 11/10092-4 - Nanozeólitas como suportes sólidos para imobilização enzimática: Síntese, caracterização de complexos nanozeólitas/enzimas e sua aplicação como catalisadores heterogêneos para produção de biodiesel via rota etílica
Beneficiário:Adriano de Vasconcellos
Linha de fomento: Bolsas no Brasil - Doutorado