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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Ethopharmacological analysis of the open elevated plus-maze in mice

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Autor(es):
Sorregotti, Tatiani [1, 2] ; Mendes-Gomes, Joyce [1] ; Rico, Javier Leonardo [1, 3] ; Rodgers, Robert John [4] ; Nunes-de-Souza, Ricardo Luiz [1, 2, 5]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, UNESP, Sch Pharmaceut Sci, Pharmacol Lab, BR-14801902 Araraquara, SP - Brazil
[2] UFSCar UNESP, Joint Grad Program Physiol Sci, BR-13565905 Sao Carlos, SP - Brazil
[3] Fdn Univ Konrad Lorenz, Lab Anim Behav, Bogota - Colombia
[4] Univ Leeds, Inst Psychol Sci, Behav Neurosci Lab, Leeds, W Yorkshire - England
[5] Univ Sao Paulo, Inst Neurosci & Behav IneC, BR-14040901 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Behavioural Brain Research; v. 246, n. 1, p. 76-85, JUN 1 2013.
Citações Web of Science: 19
Resumo

Exposure of rodents to an open elevated plus-maze (oEPM) elicits antinociception and increases plasma corticosterone levels. However, no studies have yet assessed the defensive behaviour repertoire of animals in this modified test. In Experiment 1, factor analysis was employed to characterise the behavioural profile of mice exposed to the oEPM. Experiments 2 and 3 assessed the effects of acute alprazolam (0.5-1.5 mg/kg; diazepam 0.5-1.5 mg/kg), pentylenetetrazole (10.0-30.0 mg/kg), yohimbine (2.0-6.0 mg/kg), mCPP (0.3-3.0 mg/kg), and acute and chronic fluoxetine (10.0-30.0 mg/kg) and imipramine (1.0-15.0 mg/kg) on behaviours identified in Experiment 1. The factor analyses revealed that behaviour in the oEPM can largely (77% total variance) be accounted for in terms of 3 factors: factor 1 ('depth exploration'; e.g. head-dipping on the arms), factor 2 ('cautious exploration of arms'; e.g. flat-back approach), and factor 3 ('risk assessment'; stretched attend postures - SAP). Experiments 2 and 3 showed that, over the dose range used, alprazolam selectively attenuated all measures of defensiveness. Similar, though more modest, effects were seen with diazepam. Confirming the intensity of the emotional response to the oEPM (nociceptive, endocrine and behavioural), relatively few significant behavioural changes were seen in response to the anxiogenic compounds tested. Although acute fluoxetine or imipramine treatment failed to modify behaviour in the oEPM, chronic fluoxetine (but not chronic imipramine) attenuated total flat back approach and increased head dipping outside the central square. Together, the results indicate that the oEPM induces behavioural defensive responses that are sensitive to alprazolam and chronic fluoxetine. (C) 2013 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 10/13441-7 - Análise etofarmacológica do labirinto em cruz elevado aberto para avaliar comportamentos defensivos e a antinocicepção induzida pelo medo em camundongos
Beneficiário:Tatiani Sorregotti
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 05/01988-3 - Papel das porções dorsal e ventrolateral da matéria cinzenta periaquedutal de camundongos na modulação das reações comportamentais defensivas e antinocicepção induzidas por situações aversivas
Beneficiário:Joyce Mendes Gomes Tessari
Linha de fomento: Bolsas no Brasil - Doutorado Direto