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Stress-inducible protein 1 is a cell surface ligand for cellular prion that triggers neuroprotection

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Zanata, Silvio M. ; Lopes, Marilene H. ; Mercadante, Adriana F. ; Hajj, Glaucia N. M. ; Chiarini, Luciana B. ; Nomizo, Regina ; Freitas, Adriana R. O. ; Cabral, Ana L. B. ; Lee, Kil S. ; Juliano, Maria A. ; et al
Número total de Autores: 11
Tipo de documento: Artigo Científico
Fonte: EMBO Journal; v. 21, n. 13, p. 3307-3316, July 2002.
Área do conhecimento: Ciências Biológicas - Bioquímica
Assunto(s):Neurônios   Proteínas   Príons   Proteínas PrPC

Prions are composed of an isoform of a normal sialoglycoprotein called PrPc, whose physiological role has been under investigation, with focus on the screening for ligands. Our group described a membrane 66 kDa PrPc-binding protein with the aid of antibodies against a peptide deduced by complementary hydropathy. Using these antibodies in western blots from twodimensional protein gels followed by sequencing the specific spot, we have now identified the molecule as stress-inducible protein 1 (STI1). We show that this protein is also found at the cell membrane besides the cytoplasm. Both proteins interact in a specific and high afinity manner with a Kd of 10±7 M. The interaction sites were mapped to amino acids 113±128 from PrPc and 230±245 from STI1. Cell surface binding and pull-down experiments showed that recombinant PrPc binds to cellular STI1, and co-immunoprecipitation assays strongly suggest that both proteins are associated in vivo. Moreover, PrPc interaction with either STI1 or with the peptide we found that represents the binding domain in STI1 induce neuroprotective signals that rescue cells from apoptosis. (AU)

Processo FAPESP: 99/07124-8 - Papel da proteína prion celular em processos fisiológicos e patológicos
Beneficiário:Vilma Regina Martins
Modalidade de apoio: Auxílio à Pesquisa - Temático