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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Proteomic Characterization of the Hyaluronidase (EC 3.2.1.35) from the Venom of the Social Wasp Polybia paulista

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Autor(es):
Aparecido dos Santos Pinto, Jose Roberto [1, 2] ; dos Santos, Lucilene Delazari [1, 2] ; Arcuri, Helen Andrade [2, 3] ; Dias, Nathalia Baptista [1, 2] ; Palma, Mario Sergio [1, 2]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, Sao Paulo State Univ, Inst Biosci Rio Claro, Ctr Study Social Insects, Dept Biol, Rio Claro, SP - Brazil
[2] Inst Res Immunol INCT Iii, Sao Paulo - Brazil
[3] INCOR HC FMUSP, Discipline Allergy & Immunol, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: PROTEIN AND PEPTIDE LETTERS; v. 19, n. 6, p. 625-635, JUN 2012.
Citações Web of Science: 15
Resumo

Polybia paulista wasp venom possesses three major allergens: phospholipase A(1), hyaluronidase and antigen-5. To the best of our knowledge, no hyaluronidase from the venom of Neotropical social wasps was structurally characterized up to this moment, mainly due to its reduced amount in the venom of the tropical wasp species (about 0.5% of crude venom). Four different glycoproteic forms of this enzyme were detected in the venom of the wasp Polybia paulista. In the present investigation, an innovative experimental approach was developed combining 2-D SDS-PAGE with in-gel protein digestion by different proteolytic enzymes, followed by mass spectrometry analysis under collision-induced dissociation CID) conditions for the complete assignment of the protein sequencing. Thus, the most abundant form of this enzyme in P. paulista venom, the hyaluronidase-III, was sequenced, revealing that the first 47 amino acid residues from the N-terminal region, common to other Hymenoptera venom hyaluronidases, are missing. The molecular modeling revealed that hyaluronidase-III has a single polypeptide chain, folded into a tertiary structure, presenting a central (beta/alpha)(5) core with alternation of beta-strands and alpha-helices; the tertiary structure stabilized by a single disulfide bridge between the residues Cys(189) and Cys(201). The structural pattern reported for P. paulista venom hyaluronidase-III is compatible with the classification of the enzyme as member of the family 56 of glycosidase hydrolases. Moreover, its structural characterization will encourage the use of this protein as a model for future development of ``component-resolved diagnosis{''}. (AU)

Processo FAPESP: 11/51684-1 - Biologia de sistemas como estratégia experimental para a descoberta de novos produtos naturais na fauna de artrópodes peçonhentos do Estado de São Paulo
Beneficiário:Mario Sergio Palma
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático