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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effect of varicocele on sperm function and semen oxidative stress

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Autor(es):
Blumer, Camile Garcia [1] ; Restelli, Adriana Ester [1] ; Del Giudice, Paula Toni [1] ; Soler, Thiesa Butterby [1] ; Fraietta, Renato [1] ; Nichi, Marcilio [2] ; Bertolla, Ricardo Pimenta [1] ; Cedenho, Agnaldo Pereira [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, Dept Surg, Div Urol, Human Reprod Sect, BR-04039060 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Anim Reprod, Sch Vet Med, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: BJU INTERNATIONAL; v. 109, n. 2, p. 259-265, JAN 2012.
Citações Web of Science: 53
Resumo

OBJECTIVE To assess the effect of varicocele on sperm DNA integrity, mitochondrial activity, lipid peroxidation and acrosome integrity. PATIENTS AND METHODS In all, 30 patients with a clinically diagnosed varicocele of grade II or III and 32 men without a varicocele were evaluated for sperm DNA fragmentation (comet assay), mitochondrial activity (3,3'-diaminobenzidine assay), lipid peroxidation (malondialdehyde) and acrosome integrity (fluorescent probe labelled peanut agglutinin). RESULTS The varicocele group showed fewer spermatozoa with intact DNA (grade II, P = 0.040), more cells with inactive mitochondria (class III, P = 0.001), fewer cells with active mitochondria (class I, P = 0.005) and fewer spermatozoa with intact acrosomes (P < 0.001). Finally, no significant differences were observed in lipid peroxidation levels. CONCLUSION Men with varicocele showed an increase in sperm DNA fragmentation and a reduction in mitochondrial activity and acrosome integrity. However, lipid peroxidation levels remained unchanged. (AU)

Processo FAPESP: 06/58657-1 - Efeito da varicocele na funcao dos espermatozoides.
Beneficiário:Camile Garcia Blumer
Modalidade de apoio: Bolsas no Brasil - Mestrado