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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Influence of the Ratio of Particulate Autogenous Bone Graft/Platelet-Rich Plasma on Bone Healing in Critical-Size Defects: A Histologic and Histometric Study in Rat Calvaria

Texto completo
Autor(es):
Nagata, Maria J. [1] ; Messora, Michel [1] ; Pola, Natalia [1] ; Campos, Natalia [1] ; Vieira, Rafael [2] ; Esper, Luis A. [1] ; Sbrana, Michyele [1] ; Fucini, Stephen [1] ; Garcia, Valdir [1] ; Bosco, Alvaro [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Div Periodont, Dept Surg & Integrated Clin, Dent Sch Aracatuba, BR-16015050 Aracatuba, SP - Brazil
[2] Sao Paulo State Univ, UNESP, Dept Clin Surg & Anim Reprod, Vet Sch Aracatuba, Div Clin Lab, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF ORTHOPAEDIC RESEARCH; v. 28, n. 4, p. 468-473, APR 2010.
Citações Web of Science: 22
Resumo

The purpose of this study was to analyze histomorphometrically the influence of the ratio of particulate autogenous bone (AB) graft/platelet-rich plasma (PRP) on bone healing in surgically created critical-size defects (CSD) in rat calvaria. Fifty rats were divided into five groups: Group C (control), Group AB, Group AB/PRP-50, Group AB/PRP-100, and Group AB/PRP-150. A 5-mm diameter critical-size defect was created in the calvarium of each animal. In Group C, the defect was filled by blood clot only. In Group AB, the defect was filled with 0.01 mL of AB graft. In Groups AB/PRP-50, AB/PRP-100, and AB/PRP-150, the defects were filled with 0.01 mL of AB graft combined with 50, 100, and 150 mu L of PRP, respectively. All animals were euthanized at 30 days postoperative. Histomorphometry, using image analysis software, and histology analyses were performed. New Bone Area (NBA) and the remaining bone graft particles area (RPA) were calculated as a percentage of the total area of the original defect. Percentage data were transformed into arccosine for analysis. No defect completely regenerated with bone. Group AB/PRP-50 (41.78 +/- 13.48%) had a significantly greater NBA than Groups C (19.29 +/- 5.11%), AB (27.43 +/- 10.90%) or AB/PRP-150 (19.17 +/- 8.45%) (p < 0.05). No significant differences were observed between groups AB/PRP-50 and AB/PRP-100 or among groups AB, AB/PRP-100, and AB/PRP-150 with regard to NBA (p > 0.05). Group AB/PRP-150 (31.59 +/- 3.22%) had a significantly greater EPA than Groups AB (19.09 +/- 5.21%), AB/PRP-50 (17.33 +/- 4.43%), and AB/PRP-100 (19.72 +/- 3.62%) (p < 0.001). No significant differences were observed among groups AB, AB/PRP-50, and AB/PRP-100 with regard to RPA (p > 0.05). The ratio AB graft/PRP influences bone healing in surgically created CSD in rat calvaria. (C) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:468-473, 2010 (AU)

Processo FAPESP: 06/58093-0 - Influência da proporção plasma rico em plaquetas/enxerto ósseo autógeno no reparo ósseo de defeitos de tamanho crítico em calvárias de ratos: estudo histológico e histométrico
Beneficiário:Natália Marcumini Pola
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica