Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A PCR-Based Strategy for Dombrock Screening in Brazilian Blood Donors Reveals a Novel Allele: The DO{*}A-WL

Texto completo
Autor(es):
Baleotti, Jr., Wilson [1] ; Suzuki, Rodrigo Buzinaro [1] ; Polotto, Milena [2] ; Ruiz, Marcelo Ortega [1] ; Fabron, Jr., Antonio [1] ; Castilho, Lilian [3]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] FAMEMA, Hemoctr, Fac Med, BR-17519050 Sao Paulo - Brazil
[2] FAMERP, Sao Paulo - Brazil
[3] Hemoctr Unicamp, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF CLINICAL LABORATORY ANALYSIS; v. 25, n. 2, p. 79-82, 2011.
Citações Web of Science: 4
Resumo

Background: Determination of the molecular basis underlying the antigens in the Dombrock blood group system has shown various rearrangements between the alleles associated with DO{*}A and DO{*}B. Based on this, we employed a PCR-based strategy to screen DO alleles (DO{*}A, DO{*}B, HY{*}1, HY{*}2 and JO) in Brazilians. Methods: We tested DNA of 278 Brazilian blood donors by PCR-RFLP on plates of 96 wells to determine the 793A/G (DO{*}A/DO{*}B), 323G/T (HY), 350C/T (JO) and 898C/G (HY{*}1/HY{*}2) single nucletide polymorphisms. In order to confirm the results sequence analysis was also performed. Results: When samples of these donors were analyzed, a novel allele combination, the DO{*}A allele (793A and 323G) associated with 898G was identified and designated as DO{*}A-WL allele. This new allele encoding 300Val is the same as HY{*}1 at nucleotide 898 on the molecular background of DO{*}A. Among the 556 alleles analyzed by PCR-RFLP, 3 were DO{*}A-WL and 78 were DO{*}B-WL. This represents an overall frequency of 0.5% for DO{*}A-WL and 14% for DO{*}B-WL across the population studied. Conclusion: Molecular screening of Brazilians revealed one novel allele, the DO{*}A-WL. Our data highlight the importance of testing a cohort of different populations to determine DO haplotypes and to establish reliable genotyping tests for predicting Do(a)/Do(b) status. J. Clin. Lab. Anal. 25:79-82, 2011. (C) 2011 Wiley-Liss, Inc. (AU)

Processo FAPESP: 07/59546-1 - Genotipagem dos antígenos dos sistemas de grupos sanguíneos Diego, Dombrock, Colton e Cartwright, e sua utilização na clínica transfusional
Beneficiário:Wilson Baleotti Junior
Linha de fomento: Auxílio à Pesquisa - Regular