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The potential of phenothiazinium dyes as cytotoxicity markers in cisplatin-treated cells

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Autor(es):
Pereira, Luiz Miguel ; Portapilla, Gisele Bulhoes ; Brancini, Guilherme Thomaz Pereira ; Possato, Bruna ; da Costa, Cassia Mariana Bronzon ; Abreu-Filho, Pericles Gama ; Wainwright, Mark ; Yatsuda, Ana Patricia ; Braga, Gilberto ubida Leite
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 13, n. 1, p. 17-pg., 2023-06-23.
Resumo

Assessing the in vitro toxicity of compounds on cell cultures is an important step during the screening of candidate molecules for diverse applications. Among the strategies employed to determine cytotoxicity, MTT, neutral red, and resazurin are commonly used. Methylene blue (MB), a phenothiazinium salt, has several uses, such as dye, redox indicator, and even as treatment for human disease and health conditions, such as malaria and methemoglobinemia. However, MB has only been sparsely used as a cellular toxicity indicator. As a viability indicator, MB is mostly applied to fixed cultures at high concentrations, especially when compared to MTT or neutral red. Here we show that MB and its related compounds new methylene blue (NMB), toluidine blue O (TBO), and dimethylmethylene blue (DMMB) can be used as cytotoxicity indicators in live (non-fixed) cells treated for 72 h with DMSO and cisplatin. We compared dye uptake between phenothiazinium dyes and neutral red by analyzing supernatant and cell content via visible spectra scanning and microscopy. All dyes showed a similar ability to assess cell toxicity compared to either MTT or neutral red. Our method represents a cost-effective alternative to in vitro cytotoxicity assays using cisplatin or DMSO, indicating the potential of phenothiazinium dyes for the screening of candidate drugs and other applications. (AU)

Processo FAPESP: 18/21020-3 - Fator de despolimerização de actina (NcADF): oxidação como regulador da atividade de NcADF na morte celular programada de Neospora caninum
Beneficiário:Ana Patricia Yatsuda Natsui
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/17579-2 - Avalição de novos compostos de coordenação com ligante melatonina como candidatos a metalofármacos: síntese, caracterização e estudos de atividade tripanocida
Beneficiário:Bruna Possato Dezsa
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 18/10711-5 - Avaliação do potencial de corantes fenotiazínicos para o controle de Toxoplasma gondii
Beneficiário:Luiz Miguel Pereira
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 16/11386-5 - Estudo mecanístico da percepção e da resposta a luz visível no fungo entomopatogênico Metarhizium acridum
Beneficiário:Gilberto Úbida Leite Braga
Modalidade de apoio: Auxílio à Pesquisa - Regular