Transcriptomic analysis of Malpighian tubules from... - BV FAPESP
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Transcriptomic analysis of Malpighian tubules from the stingless bee Melipona scutellaris reveals thiamethoxam-induced damages

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Autor(es):
Miotelo, Lucas ; Ferro, Milene ; Maloni, Geovana ; Otero, Igor Vinicius Ramos ; Nocelli, Roberta Cornelio Ferreira ; Bacci, Mauricio ; Malaspina, Osmar
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: Science of The Total Environment; v. 850, p. 13-pg., 2022-08-19.
Resumo

The concern about pesticide exposure to neotropical bees has been increasing in the last few years, and knowledge gaps have been identified. Although stingless bees, (e.g.: Melipona scutellaris), are more diverse than honeybees and they stand out in the pollination of several valuable economical crops, toxicity assessments with stingless bees are still scarce. Nowadays new approaches in ecotoxicological studies, such as omic analysis, were pointed out as a strategy to reveal mechanisms of how bees deal with these stressors. To date, no molecular techniques have been applied for the evaluation of target and/or non-target organs in stingless bees, such as the Malpighian tubules (Mt). Therefore, in the present study, we evaluated the differentially expressed genes (DEGs) in the Mt of M. scutellaris after one and eight days of exposure to LC50/100 (0.000543 ng a.i./mu L) of thiamethoxam (TMX). Through functional annotation analysis of four transcriptome libraries, the time course line approach revealed 237 DEGs (nine clusters) associated with carbon/ energy metabolism and cellular processes (lysosomes, autophagy, and glycan degradation). The expression profiles of Mt were altered by TMX in processes, such as detoxification, excretion, tissue regeneration, oxidative stress, apopto-sis, and DNA repair. Transcriptome analysis showed that cell metabolism in Mt was mainly affected after 8 days of ex-posure. Nine genes were selected from different clusters and validated by RT-qPCR. According to our findings, TMX promotes several types of damage in Mt cells at the molecular level. Therefore, interference of different cellular pro-cesses directly affects the health of M. scutellaris by compromising the function of Mt. (AU)

Processo FAPESP: 17/21097-3 - Interações abelha-agricultura: perspectivas para a utilização sustentável
Beneficiário:Osmar Malaspina
Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
Processo FAPESP: 21/01359-9 - Efeitos do inseticida tiametoxam em Scaptotrigona postica Latreille, 1807 (Hymenoptera, Meliponina): determinação da concentração letal média e análise da atividade enzimática
Beneficiário:Geovana Maloni da Silva
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 20/03527-3 - Expressão gênica diferencial dos transcriptomas de túbulos de Malpighi de Melipona scutellaris expostos ao tiametoxam
Beneficiário:Lucas Miotelo
Modalidade de apoio: Bolsas no Brasil - Mestrado