Busca avançada
Ano de início
Entree


The Deconstruction of the Lignocellulolytic Structure of Sugarcane Bagasse by Laccases Improves the Production of H-2 and Organic Acids

Texto completo
Autor(es):
Dionizio, Bruna Soares ; Silva Rabelo, Camila Abreu B. ; Ramos de Jesus, Hugo Cesar ; Amancio Varesche, Maria Bernadete ; Ferreira de Souza, Dulce Helena
Número total de Autores: 5
Tipo de documento: Artigo Científico
Fonte: Applied Biochemistry and Biotechnology; v. 194, n. 7, p. 22-pg., 2022-03-29.
Resumo

The production of biofuels using sugarcane bagasse (SCB) as substrate can be considered an environmentally friendly approach, due to the possibility of combining energy production with the reuse of agroindustrial wastes. This study was undertaken to explore the applicability of a new extract with the enzymes (Lac(mix)) isolated from Chaetomium cupreum for SCB pretreatment. Lac(mix) was more active at pH of 2.2 to 4 and 50 to 60 degrees C. Further, the individual and mutual effects of SCB concentration (6.6 to 23.4 g L- 1), enzyme concentration (0.066 to 0.234 U L- 1), and incubation time of the SCB with Lac(mix) (19 to 221 min) on SCB pretreatment were evaluated using a response surface methodology and central composite design. The optimized conditions were 23.4 g L- 1 SCB, 0.234 U mL(- 1) laccases, and 2.44 h resulting in 547 +/- 108 mg L- 1 of total sugars. This value agrees with the predicted value (455 +/- 41 mg L- 1) by the statistical model. Through the SCB pretreated with Lac(mix) fermentation, 96.1% more H-2 and 22.5% more organic acids were observed compared to SCB without pretreatment. Therefore, laccases improve delignification, maximizing biomass fermentation for biofuel production. (AU)

Processo FAPESP: 18/26470-7 - Influência da sacarificação do bagaço de cana-de-açúcar por lacase na produção fermentativa de biocombustíveis
Beneficiário:Camila Abreu Borges da Silva Rabelo
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 17/15455-4 - Contribuição aos estudos integrados para o controle de formigas cortadeiras - busca de novos inseticidas e fungicidas - nanoencapsulamento
Beneficiário:Hugo César Ramos de Jesus
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 18/06297-9 - Produção e caracterização de proteínas quiméricas contendo domínio catalítico quitinase e módulos para ligação em quitina
Beneficiário:Dulce Helena Ferreira de Souza
Modalidade de apoio: Auxílio à Pesquisa - Regular