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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment

Texto completo
Autor(es):
dos Santos, Danilo M. [1] ; de Annunzio, Sarah R. [2] ; Carmello, Juliana C. [3] ; Pavarina, Ana C. [3] ; Fontana, Carla R. [2] ; Correa, Daniel S. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Embrapa Instrumentacao, Nanotechnol Natl Lab Agr LNNA, BR-13560970 Sao Carlos, SP - Brazil
[2] UNESP Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Clin Anal, BR-14801903 Araraquara, SP - Brazil
[3] UNESP Sao Paulo State Univ, Sch Dent, Dept Dent Mat & Prosthodont, BR-14801903 Araraquara, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: ACS APPLIED BIO MATERIALS; DEC 2021.
Citações Web of Science: 1
Resumo

Periodontitis is a chronic inflammatory disease that can lead to significant destruction of tooth-supporting tissues, compromising dental function and patient's health. Although the currently employed treatment approaches can limit the advance of the disease, the development of multifunctional and hierarchically structured materials is still in demand for achieving successful tissue regeneration. Here, we combine coaxial electrospinning and 3D printing techniques to prepare bilayered zein-based membranes as a potential dual drug delivery platform for periodontal tissue regeneration. A layer of core-sheath electrospun nanofibers consisting of poly(ethylene oxide) (PEO)/curcumin (Curt)/tetracycline hydrochloride (TH) as the core and zein/poly(epsilon-caprolactone)(PCL)/beta-glycerolphosphate (beta-GP) as the sheath was deposited over a 3D printed honeycomb PLA/zein/Curc platform in order to render a bilayered structure that can mimic the architecture of periodontal tissue. The physicochemical properties of engineered constructs as well as the release profiles of distinct drugs were mainly controlled by varying the concentration of zein (10, 20, 30%, w/w relative to dry PCL) on the sheath layer of nanofibers, which displayed average diameters ranging from 150 to 400 nm. In vitro experiments demonstrated that the bilayered constructs provided sustained release of distinct drugs over 8 days and exhibited biocompatibility toward human oral keratinocytes (Nok-si) (cell viability >80%) as well as antibacterial activity against distinct bacterial strains induding those of the red complex such as Porphyromonas gingivalis and Treponema denticola, which are recognized to elicit aggressive and chronic periodontitis. Our study reveals the potential of zein-based bilayered membranes as a dual drug delivery platform for periodontal tissue regeneration. (AU)

Processo FAPESP: 17/20973-4 - Nanofibras eletrofiadas coaxiais à base de quitosana para a liberação controlada de antibióticos e aplicações no tratamento de lesões periodontais
Beneficiário:Danilo Martins dos Santos
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 18/09088-1 - Avaliação da terapia fotodinâmica contra biofilmes acneicos polimicrobianos: um estudo pré-clínico
Beneficiário:Sarah Raquel de Annunzio
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 18/22214-6 - Rumo à convergência de tecnologias: de sensores e biossensores à visualização de informação e aprendizado de máquina para análise de dados em diagnóstico clínico
Beneficiário:Osvaldo Novais de Oliveira Junior
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 18/23015-7 - Terapia fotodinâmica antimicrobiana via irradiação em modo contínuo e chaveado contra Enterococcus faecalis e Cutibacterium acnes
Beneficiário:Carla Raquel Fontana Mendonça
Linha de fomento: Auxílio à Pesquisa - Regular