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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Styrax camporum, a typical species of the Brazilian cerrado, attenuates DNA damage, preneoplastic lesions and oxidative stress in experimental rat colon carcinogenesis

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Autor(es):
Francielli De Oliveira, Pollyanna [1, 2] ; Leandro, Luis Fernando [2] ; Furtado, Ricardo Andrade [2] ; Ferreira, Natalia Helen [2] ; Pauletti, Patricia Mendonca [2] ; Barbosa Araujo, Alba Regina [2] ; Uyemura, Sergio Akira [3] ; Tavares, Denise Crispim [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed Alfenas, Alfenas, MG - Brazil
[2] Univ Franca, Ave Dr Armando Salles de Oliveira, 201 Parque Univ, BR-14404600 Franca, SP - Brazil
[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 84, n. 14, p. 582-592, JUL 18 2021.
Citações Web of Science: 0
Resumo

Styrax camporum Pohl, a typical species from the Brazilian cerrado, commonly known as ``benjoeiro{''}, is used to treat gastroduodenal diseases. In previous studies carried out by our research group, hydroalcoholic extract of S. camporum stems (SCHE) exhibited antigenotoxic and antiproliferative effects. For a comparative analysis of the chemopreventive effect of SCHE, the aim of this study was to investigate the influence of SCHE against carcinogen 1,2-dimethylhydrazine (DMH)-induced DNA damage and pre-neoplastic lesions in Wistar rat colon. Animals were treated orally with SCHE at 250, 500 or 1000 mg/kg body weight in conjunction with a subcutaneous injection of DMH. DNA damage was assessed using the comet assay while tpre-neoplastic lesions by aberrant crypt foci (ACF) assay. The following hepatic oxidative stress markers were determined including activities of catalase (CAT) and glutathione S-transferase (GST) as well as levels of reduced glutathione (GSH) and malondialdehyde (MDA). Treatment with SCHE was not genotoxic or carcinogenic at the highest dose tested (1000 mg/kg b.w.). The extract effectively inhibited DNA damage and pre-neoplastic lesions induced by DMH administration at all concentrations tested. Measurement of CAT, and GST activities and levels of GSH showed that SCHE did not reduce oxidative processes. In contrast, treatment with SCHE (1000 mg/kg b.w.) decreased liver MDA levels. Taken together, these findings suggested the chemopreventive effect attributed to SCHE in colon carcinogenesis, may be related to its capacity to inhibit DNA damage as well as an antioxidant action associated with its chemical constituents egonol and homoegonol. (AU)

Processo FAPESP: 11/21310-2 - ESTUDO DAS ATIVIDADES CITOTÓXICA, GENOTÓXICA E ANTIOXIDANTE DO EXTRATO HIDROALCOÓLICO DE Styrax camporum E DOS SEUS MARCADORES QUÍMICOS, EGONOL E HOMOEGONOL, E DA SUA INFLUÊNCIA SOBRE OS DANOS NO MATERIAL GENÉTICO E LESÕES PRÉ-NEOPLÁSICAS
Beneficiário:Pollyanna Francielli de Oliveira
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/13903-9 - Avaliação das atividades genotóxica e antioxidante do extrato hidroalcoólico de Styrax camporum e dos seus marcadores químicos, egonol e homoegonol, e de sua influência sobre as lesões genômicas e pré-neoplásicas
Beneficiário:Denise Crispim Tavares Barbosa
Linha de fomento: Auxílio à Pesquisa - Regular