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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Mutagenicity of silver nanoparticles synthesized with curcumin (Cur-AgNPs)

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Autor(es):
Proenca-Assuncao, Jaqueline de Cassia [1] ; Constantino, Ederson [1] ; Farias-de-Franca, Anna Paula [2] ; Nogueira, Flavia Aparecida Resende [3] ; Consonni, Silvio Roberto [4] ; Chaud, Marco Vinicius [1] ; dos Santos, Carolina Alves [2] ; Oshima-Franco, Yoko [1, 2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sorocaba UNISO, Postgrad Program Pharmaceut Sci, Sorocaba, SP - Brazil
[2] Univ Sorocaba Uniso, Pharm Grad Course, Sorocaba, SP - Brazil
[3] Univ Araraquara, Dept Biol Sci & Hlth, Araraquara, SP - Brazil
[4] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF SAUDI CHEMICAL SOCIETY; v. 25, n. 9 SEP 2021.
Citações Web of Science: 0
Resumo

Introduction: Silver nanoparticles (AgNPs) are of particular interest for their antibacterial properties and are produced by the action of reducing agents on silver ions. Curcumin from Curcuma longa (Zingiberaceae) has been used as a precursor for obtaining biogenic AgNPs, to act as a potential drug. Objectives: This study aimed to evaluate the toxicity of AgNPs synthesized with curcumin (CurAgNPs 0.081 mg/mL, similar to 130 nm) through the Salmonella/microsome (Ames test), one of the first required assays for evaluating toxicity. Methods: The study design was experimental and in vitro. After defining the preliminary toxicity, the mutagenicity was assessed in a concentration range of 0.0010-0.0081 mg/plate Cur-AgNPs using histidine negative (His-) Salmonella Typhimurium strains TA97a, TA98, TA100, and TA102, with (+S9) and without metabolic activation (-S9), in triplicate. Assays were monitored by positive and negative controls. The results were statistically analyzed by Salanal software with p < 0.05 values considered significant. Results: The data obtained in the absence of metabolic activation showed that Cur-AgNPs is not mutagenic, but when exposed to the presence of S9, Cur-AgNPs became mutagenic to TA98 and TA100 strains, showing the significance of metabolizer enzymes to activate Cur-AgNPs on these bacteria, which recovered their abilities in synthesizing histidine (His+). Conclusion: Cur-AgNPs is mutagenic in the presence (+S9), but not in the absence (- S9) of metabolic activation, being able to act as indirect mutagens potentially to organisms that share the same genotype vulnerabilities found in TA98 and TA100 strains to cause a frameshift and base-pair substitution mutations, respectively. (C) 2021 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. (AU)

Processo FAPESP: 19/05891-7 - Influência de nanopartículas de prata (AgNPs) sobre o bloqueio neuromuscular in vitro do veneno da serpente Philodryas olfersii (Dipsadidae)
Beneficiário:Anna Paula Farias de França
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 12/08271-0 - IDENTIFICAÇÃO E FARMACODINÂMICA DE FRAÇÕES NEUROFACILITADORAS DA Casearia sylvestris SW. NA JUNÇÃO NEUROMUSCULAR
Beneficiário:Yoko Oshima Franco
Linha de fomento: Auxílio à Pesquisa - Regular