Bacterial diversity in organic and conventional Mi... - BV FAPESP
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Bacterial diversity in organic and conventional Minas Frescal cheese production using targeted 16S rRNA sequencing

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Autor(es):
da Silva Abreu, Anderson Clayton [1] ; Carazzolle, Marcelo Falsarella [2] ; Crippa, Bruna Lourenco [1] ; Barboza, Giovana Rueda [1] ; Rall, Vera Lucia Mores [3] ; Rocha, Liliana de Oliveira [1] ; Cirone Silva, Nathalia Cristina [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Sch Food Engn FEA, Dept Food Sci, BR-13083862 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol IB, Dept Genet & Mol Biol, BR-13083862 Campinas, SP - Brazil
[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Chem & Biol Sci, BR-18618691 Botucatu, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: INTERNATIONAL DAIRY JOURNAL; v. 122, NOV 2021.
Citações Web of Science: 0
Resumo

Molecular identification and the study of bacteria in processed food products can be challenging or suffer limitations when using conventional methods. However, with Next Generation Sequencing (NGS), one can identify bacterial species with a significantly higher detection sensitivity using a 16S rRNA target gene. This study aimed to characterise the bacterial diversity of the organic and conventional production of Minas Frescal cheese, through the NGS analysis of the 16S rRNA gene. DNA was extracted from 96 samples, 48 from organic production and 48 from conventional production. The bacterial families and genera with the highest prevalence were identified. The most prevalent families were Enterobacteriaceae, Planococcaceae and Moraxellaceae. The NGS 16S rRNA tool proved to be very useful for bacterial characterisation, but it is still not possible to say whether a product contains more or less bacteria based on organic production, as there are several factors that contribute to contamination. (c) 2021 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 16/02118-7 - Ferramentas de biologia molecular para estudo da diversidade microbiana e caracterização de Staphylococcus aureus em laticínios orgânicos e convencionais no Estado de São Paulo
Beneficiário:Nathalia Cristina Cirone Silva
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/00986-7 - Staphylococcus coagulase negativa: análise do perfil de resistência e enterotoxigênico em cepas isoladas de laticínios
Beneficiário:Luiz Gustavo de Matos
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica