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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Omega-3 Supplementation Prevents Short-Term High-Fat Diet Effects on the alpha 7 Nicotinic Cholinergic Receptor Expression and Inflammatory Response

Texto completo
Autor(es):
Martins, I. C. A. [1] ; Contieri, L. S. [1] ; Amaral, C. L. [1] ; Costa, S. O. [1] ; Souza, A. C. P. [1] ; Ignacio-Souza, L. M. [2, 1] ; Milanski, M. [2, 1] ; Torsoni, A. S. [2, 1] ; Torsoni, M. A. [2, 1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Sch Appl Sci, Lab Metab Disorders, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Obes & Comorbid Res Ctr, Campinas, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Mediators of Inflammation; v. 2021, AUG 10 2021.
Citações Web of Science: 0
Resumo

The study is aimed at investigating if PUFA supplementation could prevent the effects of a short-term HFD on alpha 7nAChR expression and on the severity of sepsis. Swiss mice were used for the in vivo experiments. For the in vitro experiments, we used a microglia cell line (BV-2) and a hepatoma cell line (Hepa-1c1c7) derived from mice. The animals were either fed standard chow, fed a short-term HFD (60%), or given supplementation with omega-3 fatty acid (2 g/kg or 4 g/kg bw) for 17 days, followed by a short-term HFD. Endotoxemia was induced with an intraperitoneal (i.p.) lipopolysaccharide injection (LPS, 5 or 12 mg/kg), and sepsis was induced by subjecting the animals to cecal ligation and puncture (CLP). BV-2 and Hepa-1c1c7 cells were treated with LPS (100 and 500 ng/mL, respectively) for 3 hours. RT-PCR or Western blotting was used to evaluate alpha 7nAChR expression, inflammatory markers, DNMT1, and overall ubiquitination. LPS and HFD reduced the expression of alpha 7nAChR and increased the expression of inflammatory markers. Omega-3 partially prevented the damage caused by the HFD to the expression of alpha 7nAChR in the bone marrow and hypothalamus, decreased the inflammatory markers, and reduced susceptibility to sepsis-induced death. Exposing the BV-2 cells to LPS increased the protein content of DNMT1 and the overall ubiquitination and reduced the expression of alpha 7nAChR. The inflammation induced by LPS in the BV-2 cell decreased alpha 7nAChR expression and concomitantly increased DNMT1 expression and the ubiquitinated protein levels, indicating the participation of pre- and posttranscriptional mechanisms. (AU)

Processo FAPESP: 16/23484-1 - Participação de receptores colinérgicos do tipo nicotínico alfa7 (alfa7nAChR) na prevenção do desenvolvimento de resistência à insulina
Beneficiário:Marcio Alberto Torsoni
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/01863-6 - Papel do receptor nicotínico colinérgico alfa 7 (alfa7nAChR) no controle hipotalâmico da homeostase energética e associação com a sinalização da leptina em camundongos
Beneficiário:Camilla Mendes de Souza
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 13/07607-8 - CMPO - Centro Multidisciplinar de Pesquisa em Obesidade e Doenças Associadas
Beneficiário:Licio Augusto Velloso
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 19/07615-7 - Papel do receptor nicotínico colinérgico ±7 (±7nAChR) no controle hipotalâmico da homeostase energética e associação com a sinalização central da leptina em camundongos
Beneficiário:Marcio Alberto Torsoni
Linha de fomento: Auxílio à Pesquisa - Regular