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Enzymatic responses in the head and midgut of Africanized Apis mellifera contaminated with a sublethal concentration of thiamethoxam

Texto completo
Autor(es):
Decio, Pamela [1] ; Miotelo, Lucas [1] ; Pereira, Franco Dani Campos [1, 2] ; Roat, Thaisa Cristina [1] ; Marin-Morales, Maria Aparecida [1] ; Malaspina, Osmar [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ Unesp, Inst Biosci, Ave 24A, 1515, BR-13506900 Rio Claro, SP - Brazil
[2] NUPEFEN Nucleo Pesquisas Educ Fis Estet & Nutr, Claretiano Univ Ctr, Ave Santo Antonio Maria Claret, BR-13503257 Rio Claro, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 223, OCT 15 2021.
Citações Web of Science: 0
Resumo

The increasing use of insecticides, promoted by the intensification of agriculture, has raised concerns about their influence on the decline of bee colonies, which play a fundamental role in pollination. Thus, it is fundamental to elucidate the effects of insecticides on bees. This study investigated the damage caused by a sublethal concentration of thiamethoxam - TMX (0.0227 ng/mu L of feed) in the head and midgut of Africanized Apis mellifera, by analyzing the enzymatic biomarkers, oxidative stress, and occurrence of lipid peroxidation. The data showed that the insecticide increased acetylcholinesterase activity (AChE) and glutathione-S-transferase (GST), whereas carboxylesterase (CaE3) activity decreased in the heads. Our results indicate that the antioxidant enzymes were less active in the head because only glutathione peroxidase (GPX) showed alterations. In the midgut, there were no alkaline phosphatase (ALP) or superoxide dismutase (SOD) responses and a decrease in the activity of CaE was observed. Otherwise, there was an increase in GPX, and the TBARS (thiobarbituric acid reactive substances) assay also showed differences in the midgut. The TBARS (thiobarbituric acid reactive substances) assay also showed differences in the midgut. The results showed enzymes such as CaE3, GST, AChE, ALP, SOD, and GPX, as well as the TBARS assay, are useful biomarkers on bees. They may be used in combination as a promising tool for characterizing bee exposure to insecticides. (AU)

Processo FAPESP: 13/21833-0 - Proteção de polinizadores: toxicidade do tiametoxam para Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini)
Beneficiário:Lucas Miotelo
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 17/21097-3 - Interações abelha-agricultura: perspectivas para a utilização sustentável
Beneficiário:Osmar Malaspina
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 14/23197-7 - Estresse celular e atividade de enzimas biomarcadoras em abelhas africanizadas Apis mellifera Lineu, 1758 (Hymenoptera, Apidae) expostas ao tiametoxam
Beneficiário:Pâmela Decio Horst
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 12/13370-8 - Como o cérebro de Apis mellifera (Lineu, 1758) (Hymenoptera, Apidae) responde à dose subletal de tiametoxam?
Beneficiário:Thaisa Cristina Roat
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores