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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes

Texto completo
Autor(es):
Barbosa, Fernando Cesar [1] ; Kendrick, Emanuele [2] ; Brenelli, Livia Beatriz [3, 4] ; Arruda, Henrique Silvano [5] ; Pastore, Glaucia Maria [5] ; Rabelo, Sarita Candida [6] ; Damasio, Andre [7] ; Franco, Telma Teixeira [3, 8] ; Leak, David [2] ; Goldbeck, Rosana [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Campinas UNICAMP, Sch Food Engn, Bioproc & Metab Engn Lab, Campinas, SP - Brazil
[2] Univ Bath, Fac Sci, Dept Biol & Biochem, Bath, Avon - England
[3] Univ Estadual Campinas, Interdisciplinary Ctr Energy Planning, Campinas, SP - Brazil
[4] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas, SP - Brazil
[5] Univ Estadual Campinas, Sch Food Engn, Dept Food Sci, Bioflavors & Bioact Cpds Lab, BR-13083862 Campinas, SP - Brazil
[6] Sao Paulo State Univ UNESP, Coll Agr Sci, Dept Bioproc & Biotechnol, Botucatu, SP - Brazil
[7] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, BR-13083862 Campinas, SP - Brazil
[8] Univ Campinas UNICAMP, Fac Chem Engn, Lab Biochem Engn Biorefining & Prod Renewable Ori, Campinas, SP - Brazil
Número total de Afiliações: 8
Tipo de documento: Artigo Científico
Fonte: BIOMASS & BIOENERGY; v. 141, OCT 2020.
Citações Web of Science: 7
Resumo

Enzymatic hydrolysis of lignocellulosic biomass accounts for 20-30% of the total cost of second-generation bioethanol production and many efforts have been made in recent years to overcome the high cost of enzymes. Using cello-oligosaccharides (COS), intermediates in cellulose conversion to glucose, may provide advantages over monomeric glucose fermentation, such as lower risk of growth of process contaminants, shorter fermentation time and limited process inhibition by high concentrations of glucose. In addition, COS are also useful as functional oligosaccharides in the food and feed sectors. This study aimed to optimize COS production for further industrial applications. To the best of our knowledge, this is the first study that has used a design of experiments approach to analyze the synergism between endoglucanases, lytic polysaccharide monooxygenase (LPMO), cellobiose dehydmgenase (CDH) and different additives during the hydrolysis of a pretreated sugarcane straw for COS production. After optimization of enzymatic hydrolysis, a combination of the endoglucanases CaCel and CcCel9m, the LPMO TrCe161A, the CDH NcCDHIIa, with lactose and copper as additives, produced 60.49 mg of COS per g of pretreated sugarcane straw, 1.8-2.7-fold more than the commercial enzyme cocktails Cellic (R) Ctec2 and Celluclast (R) 1.5 L. The COS/glucose ratio achieved was 298.31, an increase of 3314 and 2294-fold over the commercial enzymatic cocktails, respectively. These results open a new perspective regarding COS production and its industrial application. (AU)

Processo FAPESP: 17/16159-0 - Otimização da produção de celo-oligossacarídeos a partir da hidrólise enzimática da palha da cana-de-açúcar via expressão heteróloga de enzimas celulolíticas e oxidativas
Beneficiário:Fernando César Barbosa
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 19/08542-3 - Biotecnologia enzimática para produção de xilo-oligossacarídeos a partir de resíduos agroindustriais lignocelulósicos visando a produção de simbióticos
Beneficiário:Rosana Goldbeck Coelho
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/22669-0 - N-glicosilação e secreção de enzimas em fungos filamentosos
Beneficiário:André Ricardo de Lima Damasio
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular
Processo FAPESP: 15/50612-8 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks
Beneficiário:Telma Teixeira Franco
Linha de fomento: Auxílio à Pesquisa - Temático