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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Osteoclast formation, inflammation, and matrix metalloproteinase-9 are downregulated in bone repair following root canal treatment in dogs teeth

Texto completo
Autor(es):
Garcia Paula-Silva, Francisco Wanderley [1] ; Manfrin Arnez, Maya Fernanda [1] ; Chaves Lamarque, Giuliana de Campos [1] ; Petille, Renato [1] ; Ribeiro-Santos, Fernanda Regina [1, 2] ; de Sena, Marina Fernandes [1] ; Nelson-Filho, Paulo [1] ; Bezerra da Silva, Lea Assed [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Clin, Ribeirao Preto, SP - Brazil
[2] Univ Pernambuco, Arco Verde, PE - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: CLINICAL ORAL INVESTIGATIONS; v. 25, n. 7, p. 4699-4707, JUL 2021.
Citações Web of Science: 0
Resumo

Objectives The aim of this study was to investigate the inflammatory infiltrate, osteoclast formation, and expression of MMP-9 during the healing phase following root canal treatment in teeth with apical periodontitis. Materials and methods Apical periodontitis was induced in dogs teeth, and root canal treatment was performed in a single visit or using calcium hydroxide as intracanal medication. One hundred and eighty days following treatment the presence of inflammation was examined, and the tissues were stained to detect osteoclasts by means of a tartrate resistant alkaline phosphatase (TRAP) assay. Synthesis of MMP-9 was detected using Western blotting and immunohistochemistry. Results Teeth with apical periodontitis that had root canal therapy performed in a single visit presented a higher synthesis of MMP-9 compared with root canal treatment using calcium hydroxide. Treatment with calcium hydroxide resulted in a reduced amount of inflammatory cells and MMP-9 positive cells. Osteoclast formation, the number of MMP-9 positive osteoclasts and cementocytes, was reduced following root canal treatment, regardless of the root canal treatment protocol used. Conclusion Root canal treatment reduced the amount of inflammatory cells and osteoclasts in periapical area. The use of calcium hydroxide as intracanal medication resulted in a lower synthesis of MMP-9, though the number of osteoclasts and MMP-9 positive osteoclasts were similar between the groups. (AU)

Processo FAPESP: 09/16882-7 - Osteoclastogênese e expressão de metaloproteinase da matriz-9 na reparação apical e periapical pós-tratamento de canais radiculares
Beneficiário:Renato Petille
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 19/02060-7 - American Academy of Pediatric Dentistry 72nd Annual Session
Beneficiário:Francisco Wanderley Garcia de Paula e Silva
Linha de fomento: Auxílio à Pesquisa - Reunião - Exterior