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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Orodispersible Film Loaded with Enterococcus faecium CRL183 Presents Anti-Candida albicans Biofilm Activity In Vitro

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Autor(es):
Lordello, Virginia Barreto [1] ; Meneguin, Andreia Bagliotti [1] ; de Annunzio, Sarah Raquel [1] ; Pia Taranto, Maria [2] ; Chorilli, Marlus [1] ; Fontana, Carla Raquel [1] ; Umbelino Cavallini, Daniela Cardoso [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, UNESP, Sch Pharmaceut Sci, Rodovia Araraquara Jau, Km 01-S-N, Campus Ville, BR-14800903 Araraquara, SP - Brazil
[2] Consejo Nacl Invest Cient & Tecn, Reference Ctr Lactobacilli CERELA, Chacabuco 145, T4000 ILC, San Miguel De Tucuman - Argentina
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: PHARMACEUTICS; v. 13, n. 7 JUL 2021.
Citações Web of Science: 0
Resumo

Background: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (>9 log(10) CFU center dot g(-1)), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation. (AU)

Processo FAPESP: 18/09088-1 - Avaliação da terapia fotodinâmica contra biofilmes acneicos polimicrobianos: um estudo pré-clínico
Beneficiário:Sarah Raquel de Annunzio
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 18/23015-7 - Terapia fotodinâmica antimicrobiana via irradiação em modo contínuo e chaveado contra Enterococcus faecalis e Cutibacterium acnes
Beneficiário:Carla Raquel Fontana Mendonça
Linha de fomento: Auxílio à Pesquisa - Regular