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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Cytotoxicity and Inflammatory Mediators Release by Macrophages Exposed to Real Seal XT and Sealapex Xpress

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Autor(es):
Léa Assed Bezerra da Silva [1] ; Lídia Regina da Costa Hidalgo [2] ; Manoel Damião de Sousa-Neto [3] ; Maya Fernanda Manfrin Arnez [4] ; Frederic Barnett [5] ; Patricia María Gatón Hernández [6] ; Lúcia Helena Faccioli ; Francisco Wanderley Garcia Paula-Silva
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Universidade de São Paul. School of Dentistry of Ribeirão Preto. Department of Paediatric Clinics - Brasil
[2] Universidade de São Paul. School of Dentistry of Ribeirão Preto. Department of Paediatric Clinics - Brasil
[3] Universidade de São Paul. School of Dentistry of Ribeirão Preto. Department of Paediatric Clinics - Brasil
[4] Universidade de São Paul. School of Dentistry of Ribeirão Preto. Department of Paediatric Clinics - Brasil
[5] Albert Einstein Medical Center. Department of Dental Medicine - Estados Unidos
[6] Universitat de Barcelona - Espanha
Número total de Afiliações: 8
Tipo de documento: Artigo Científico
Fonte: Brazilian Dental Journal; v. 32, n. 1, p. 48-52, 2021-04-02.
Resumo

Abstract This study evaluated the cytotoxicity of Sealapex Xpress and Real Seal XT and their effect on macrophage activation. J774.1 macrophages were incubated with Sealapex Xpress and Seal Real XT (0.1, 1.0, and 10 mg/mL) for 24 and 48 h. Cell viability was assessed by the MTT assay and macrophage activation was measured by pro- and anti-inflammatory cytokine production using ELISA. Data were analyzed using one-way ANOVA and Tukey’s post-test (a=0.05). Cell viability was not affected with 0.1 or 1.0 mg/mL of extracts of Sealapex Xpress and Real Seal XT at 24 and 48 h (p>0.05), but was significantly lower when cells were exposed to 10 mg/mL of both sealers (p<0.05). Sealapex Xpress inhibited the production of TNF-a, whereas Real Seal XT induced TNF-a secretion at 24 h (p<0.05). IL-6 production was induced by Real Seal XT, but not by Sealapex Xpress (p<0.05). Real Seal XT and Sealapex Xpress induced the secretion of anti-inflammatory IL-10. IL-4 was not detected in any group. In conclusion, both sealers had low toxicity but differentially activated macrophages. Macrophage activation by Sealapex Xpress was characterized by inhibition of TNF-a and induction of IL-10, whereas Real Seal XT induced IL-6 solely. (AU)

Processo FAPESP: 19/00204-1 - Papel do fator de necrose tumoral-alfa na inflamação e no reparo pulpar e periapical
Beneficiário:Francisco Wanderley Garcia de Paula e Silva
Modalidade de apoio: Auxílio à Pesquisa - Regular