RNA sequencing data of different grade astrocytoma... - BV FAPESP
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RNA sequencing data of different grade astrocytoma cell lines

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Autor(es):
de Sousa, Juliana Ferreira [1] ; da Silva, Patrick [2] ; Serafim, Rodolfo Bortolozo [2, 3, 4] ; Nociti, Ricardo Perecin [3, 4, 5] ; Moreira, Cristiano Gallina [2] ; Silva, Wilson Araujo [6, 3, 4, 7] ; Valente, Valeria [2, 3, 4]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] NCI, Radiat Oncol Branch, 10 Ctr Dr, Bethesda, MD 20892 - USA
[2] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Clin Anal, Rodovia Araraquara Jau, Km 01 S-N, BR-14800903 Araraquara, SP - Brazil
[3] Natl Inst Sci & Technol Stem Cell & Cell Therapy, Rua Tenente Catao Roxo 2501, BR-14051140 Ribeirao Preto, SP - Brazil
[4] Ctr Cell Based Therapy, Rua Tenente Catao Roxo 2501, BR-14051140 Ribeirao Preto, SP - Brazil
[5] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Ave Duque Caxias Norte 225, Campus Fernando Costa, BR-13635900 Pirassununga, SP - Brazil
[6] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil
[7] Univ Sao Paulo, NAP, CISBi, Ctr Integrat Syst Biol, Rua Catao Roxo 2501, BR-14051140 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: DATA IN BRIEF; v. 34, FEB 2021.
Citações Web of Science: 0
Resumo

Astrocytomas are the most common and aggressive type of primary brain tumors in adults. The World Health Organization (WHO) assorts them into grades, from I to IV, based on histopathological features that reflect their malignancy {[}1]. Alongside with tumor progression, comes an increased proliferation, genomic instability, infiltration in normal brain tissue and resistance to treatments. The high genomic instability forges tumor cells enhancing key proteins that avoid cells from collapsing and favor therapy resistance {[}2]. To explore genes and pathways associated with tumor progression phenotypes we analyzed gene expression in a panel of non-tumor and glioma cell lines, namely: ACBRI371, non-tumor human astrocytes; HDPC, fibroblasts derived from dental pulp; Res186, Res259, Res286 and UW467 that include grade I, II and III astrocytoma cell lines derived from pediatric tumors; and T98G, U343MG, U87MG, U138MG and U251MG, all derived from GBM (grade IV). We also profiled gene expression changes caused by exogenously induced replicative stress, performing RNA sequencing with camptothecin (CPT)-treated cells. Here we describe the RNA-sequencing data set acquired, including quality of reads and sequencing consistency, as well as the bioinformatics strategy used to analyze it. We also compared gene expression patterns and pathway enrichment between non-tumor versus lower-grade (LGG), non-tumor versus GBM, LGG versus GBM, and CPT-treated versus non-treated cells. In brief, a total of 6467 genes showed differential expression and 5 pathways were enriched in tumor progression, while 2279 genes and 7 pathways were altered under the replication stress condition. The raw data was deposited in the NCBI BioProject database under the accession number PRJNA631805. Our dataset is valuable for researchers interested in differential gene expression among different astrocytoma grades and in expression changes caused by replicative stress, facilitating studies that seek novel biomarkers of glioma progression and treatment resistance. (C) 2020 Published by Elsevier Inc. (AU)

Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
Beneficiário:Dimas Tadeu Covas
Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 13/13465-1 - Caracterização funcional da HJURP (Holliday junction recognizing protein) em células de glioblastoma multiforme
Beneficiário:Valeria Valente
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/05018-9 - Investigação do mecanismo de ação da HJURP (Holliday Junction Recognizing Protein) no reparo de DNA em células de glioblastoma
Beneficiário:Valeria Valente
Modalidade de apoio: Auxílio à Pesquisa - Regular