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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Rutin present in Alibertia edulis extract acts on human platelet aggregation through inhibition of cyclooxygenase/thromboxane

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Autor(es):
Lescano, Caroline Honaiser [1] ; de Lima, Fernando Freitas [2] ; Cardoso, Claudia Andrea Lima [3] ; Vieira, Silvia Cristina Heredia [4] ; Monica, Fabiola Zakia [1] ; de Oliveira, Ivan Pires [5]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Fac Med Sci, Dept Pharmacol, Campinas - Brazil
[2] Inst Food Technol, Ctr Dairy Technol, Campinas - Brazil
[3] Univ Mato Grosso, Ctr Nat Resource Studies, Dourados, MS - Brazil
[4] Anhanguera Uniderp Univ, Environm & Reg Dev Postgrad Program, Campo Grande, MS - Brazil
[5] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: FOOD & FUNCTION; v. 12, n. 2, p. 802-814, JAN 21 2021.
Citações Web of Science: 0
Resumo

Alibertia edulis leaf extract is commonly used in folk medicine, with rutin caffeic and vanillic acids being its major compounds. The Alibertia edulis leaf extract was investigated for its pharmacological effects via platelet aggregation, calcium mobilization, cyclic nucleotides levels, vasodilator-stimulated phosphoprotein Ser157 and Ser239 and protein kinase C beta(2) phosphorylation, thromboxane B-2, cyclooxygenases 1 and 2, docking and molecular dynamics. Alibertia edulis leaf extract significantly inhibited (100-1000 mu g mL(-1)) platelet aggregation induced by different agonists. Arachidonic acid increased levels of calcium and thromboxane B-2, phosphorylation of vasodilator-stimulated phosphoprotein Ser157 and Ser239, and protein kinase C beta, which were significantly reduced by Alibertia edulis leaf extract, rutin, and caffeic acid as well mixtures of rutin/caffeic acid. Cyclooxygenase 1 activity was inhibited for Alibertia edulis leaf extract, rutin and caffeic acid. These inhibitions were firsrtly explored by specific stabilization of rutin and caffeic acid compared to diclofenac at the catalytic site from docking score and free-energy dissociation profiles. Then, simulations detailed the rutin interactions close to the heme group and Tyr385, responsible for catalyzing the conversion of arachidonic acid to its products. Our results reveal the antiplatelet aggregation properties of Alibertia edulis leaf extract, rutin and caffeic acid providing pharmacological information about its origin from cyclooxygenase 1 inhibition and its downstream pathway. (AU)

Processo FAPESP: 17/26687-3 - Caracterização farmacológica das gliflozinas em plaquetas humanas isoladas: avaliação in vitro, in vivo e modelagem molecular
Beneficiário:Caroline Honaiser Lescano
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 18/12121-0 - Desenvolvimento de uma formulação de liberação sustentada para tetracaína em lipossomas com gradiente iônico
Beneficiário:Fernando Freitas de Lima
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 17/02201-4 - Estudo da inibição do acúmulo de nucleotídeos cíclicos por derivados de piridopirimidinas usando simulações de dinâmica molecular
Beneficiário:Ivan Pires de Oliveira
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 20/01237-8 - Encapsulação de componentes bioativos e probióticos por método combinado para desenvolvimento de bebidas funcionais à base de frutas e soro de leite
Beneficiário:Fernando Freitas de Lima
Linha de fomento: Bolsas no Brasil - Pós-Doutorado