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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Multivariate probing of antitumor metal-based complexes damage on living cells through Raman imaging

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Autor(es):
Mamian-Lopez, Monica Benicia [1, 2] ; Miguel, Rodrigo Bernardi [1] ; Araki, Koiti [1] ; Temperini, Marcia L. A. [1] ; da Costa Ferreira, Ana Maria [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[2] Fed Univ ABC, Av Estados 5001, BR-09210580 Santo Andre, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 244, JAN 5 2021.
Citações Web of Science: 0
Resumo

Intracellular modifications caused by two metal-based antitumor compounds were assessed by confocal Raman imaging assisted by multivariate curve resolution method, a very powerful deconvolution tool that can be used to extract the characteristic spectral profile of the individual or ``purest{''} components from an image dataset. The use of this Raman methodology has the advantage of being non-invasive and totally label-free. Four main different intracellular processes were observed under the Raman imaging and multivariate approach combination, and even, significant differences could be identified between the treatments with both metallodrugs. Leakage of the nucleus and nucleolus content into the cytoplasm, along with releasing of cytochrome c were observed for the treatment with the Cu-based complex. At the same time, changes of hydrogen-bonding network were also evidenced, indicating an apoptotic cellular death process, consistent with complementary Total Reflection X-Ray fluorescence (TXRF) and fluorescence experiments attesting mitochondria and DNA as main targets after uptake of the complex by cells. For treatment with the Zn-based complex, changes associated with cytochrome c were not detected, neither a rapid leakage of nucleus content upon 24 h treatment. The hydrogen-bonding network also followed a quite different pattern, suggesting that with this metallodrug, the cellular death follows a different mechanism. (C) 2020 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 14/07841-3 - Estudo das flutuações temporais e espectrais nos espectros SERS em regime de uma molécula
Beneficiário:Mónica Benicia Mamián López
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 16/21070-5 - Espectroscopia vibracional com resolução espacial
Beneficiário:Mauro Carlos Costa Ribeiro
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 12/13119-3 - Espectroscopia vibracional em fases condensadas
Beneficiário:Mauro Carlos Costa Ribeiro
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 18/21489-1 - Nanotecnologia supramolecular: design, materiais e dispositivos
Beneficiário:Henrique Eisi Toma
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 13/07937-8 - Redoxoma
Beneficiário:Ohara Augusto
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs