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Solventless separation of underivatized biogenic amines by sequential injection chromatography

Texto completo
Gama, Mariana R. [1, 2] ; Rocha, Fabio R. P. [1]
Número total de Autores: 2
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Ctr Nucl Energy Agr, Ave Centenario 303, BR-13400970 Piracicaba, SP - Brazil
[2] Univ Fed Rio Grande do Sul, Ave Bento Goncalves 9500, BR-91540000 Porto Alegre, RS - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Microchemical Journal; v. 156, JUL 2020.
Citações Web of Science: 1

Biogenic amines (BA) are markers of food freshness and indicators of inadequate food processing and storage conditions. Because BA determination is important for food quality evaluation, and considering the relatively complex analytical procedures available, new approaches are required for their determination. In this work, an environmentally friendly analytical procedure is proposed for BA determination in cheese, involving separation by sequential injection chromatography without organic solvents or chemical derivatization of the analytes. Sample preparation step was carried out by acid protein precipitation and low-temperature partition with a reduced volume of organic solvent (500 mu L of acetonitrile). Because of the previously mentioned characteristics, an analytical eco-scale = 91 was achieved. Histamine, tyramine, and tryptamine were separated in a cyanopropyl monolithic column in the isocratic elution mode, with 3.0 mmol L-1 phosphoric acid aqueous solution as mobile phase. The analytes were quantified from their intrinsic absorption at 210 nm. The analysis time was less than 3 min, with good chromatographic efficiency (N >= 75000 plates m(-1)). For the three analytes, LOD within 0.05-0.10 mg kg(-1), LOQ within 0.14-0.30 mg kg(-1), and linear response ranges up to 10 mg kg(-1) (histamine and tyramine) and 15 mg kg(-1) (tryptamine) were achieved with good precision (CV = 3.0%, n = 12). The proposed procedure was successfully applied to the determination of tyramine in cheese samples, with recoveries in the 81-128% range. (AU)

Processo FAPESP: 17/08611-0 - Avanços em cromatografia por injeção sequencial: cromatografia bidimensional e desenvolvimento de novas fases estacionárias
Beneficiário:Mariana Roberto Gama Sato
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado