Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Circulating lymphocytes and monocytes transcriptomic analysis of patients with type 2 diabetes mellitus, dyslipidemia and periodontitis

Texto completo
Autor(es):
Mostrar menos -
Corbi, Samia C. T. [1, 2] ; de Vasconcellos, Jaira F. [3, 4, 5] ; Bastos, Alliny S. [1, 6] ; Bussaneli, Diego Girotto [2] ; da Silva, Barbara Roque [2] ; Santos, Raquel Alves [7] ; Takahashi, Catarina S. [8, 9] ; Rocha, Cristiane de S. [10] ; Carvalho, Benilton de Sa [11] ; Maurer-Morelli, V, Claudia ; Orrico, Silvana R. P. [1, 6, 12] ; Barros, Silvana P. [13] ; Scarel-Caminaga, Raquel M. [2]
Número total de Autores: 13
Afiliação do(s) autor(es):
Mostrar menos -
[1] UNESP Sao Paulo State Univ, Sch Dent Araraquara, Dept Diag & Surg, BR-14801385 Araraquara, SP - Brazil
[2] UNESP Sao Paulo State Univ, Sch Dent Araraquara, Dept Morphol Genet Orthodont & Pediat Dent, BR-14801385 Araraquara, SP - Brazil
[3] NIDDK, Mol Genom & Therapeut Sect, Genet Dev & Dis Branch, NIH, 10 Ctr Dr, Bldg 10, Room 9D11, Bethesda, MD 20892 - USA
[4] Uniformed Serv Univ Hlth Sci, Dept Surg, Bethesda, MD 20814 - USA
[5] Henry Jackson Fdn Adv Mil Med, Bethesda, MD - USA
[6] Orrico, Silvana R. P., Union Coll Great Lakes UNILAGO, Adv Res Ctr Med, BR-15030070 Sao Jose Do Rio Preto, SP, Brazil.Corbi, Samia C. T., UNESP Sao Paulo State Univ, Sch Dent Araraquara, Dept Diag & Surg, BR-14801385 Araraquara, SP - Brazil
[7] Univ Franca, Postgrad Program Sci, BR-14404600 Franca, SP - Brazil
[8] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14049900 Ribeirao Preto, SP - Brazil
[9] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Biol, BR-14049900 Ribeirao Preto, SP - Brazil
[10] V, Univ Campinas UNICAMP, Dept Med Genet & Med Genom, BR-13083887 Campinas, SP - Brazil
[11] Univ Estadual Campinas, Dept Stat, Inst Math Stat & Sci Comp, BR-13083859 Campinas, SP - Brazil
[12] Union Coll Great Lakes UNILAGO, Adv Res Ctr Med, BR-15030070 Sao Jose Do Rio Preto, SP - Brazil
[13] Univ N Carolina, Dept Periodontol, Sch Dent, Chapel Hill, NC 27515 - USA
Número total de Afiliações: 13
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 MAY 18 2020.
Citações Web of Science: 0
Resumo

Type 2 diabetes mellitus (T2DM), dyslipidemia and periodontitis are frequently associated pathologies; however, there are no studies showing the peripheral blood transcript profile of these combined diseases. Here we identified the differentially expressed genes (DEGs) of circulating lymphocytes and monocytes to reveal potential biomarkers that may be used as molecular targets for future diagnosis of each combination of these pathologies (compared to healthy patients) and give insights into the underlying molecular mechanisms of these diseases. Study participants (n=150) were divided into groups: (H) systemically and periodontal healthy (control group); (P) with periodontitis, but systemically healthy; (DL-P) with dyslipidemia and periodontitis; (T2DMwell-DL-P) well-controlled type 2 diabetes mellitus with dyslipidemia and periodontitis; and (T2DMpoorly-DL-P) poorly-controlled type 2 diabetes mellitus with dyslipidemia and periodontitis. We preprocessed the microarray data using the Robust Multichip Average (RMA) strategy, followed by the RankProd method to identify candidates for DEGs. Furthermore, we performed functional enrichment analysis using Ingenuity Pathway Analysis and Gene Set Enrichment Analysis. DEGs were submitted to pairwise comparisons, and selected DEGs were validated by quantitative polymerase chain reaction. Validated DEGs verified from T2DMpoorly-DL-P versus H were: TGFB1I1, VNN1, HLADRB4 and CXCL8; T2DMwell-DL-P versus H: FN1, BPTF and PDE3B; DL-P versus H: DAB2, CD47 and HLADRB4; P versus H: IGHDL-P, ITGB2 and HLADRB4. In conclusion, we identified that circulating lymphocytes and monocytes of individuals simultaneously affected by T2DM, dyslipidemia and periodontitis, showed an altered molecular profile mainly associated to inflammatory response, immune cell trafficking, and infectious disease pathways. Altogether, these results shed light on novel potential targets for future diagnosis, monitoring or development of targeted therapies for patients sharing these conditions. (AU)

Processo FAPESP: 07/08362-8 - Avaliação da correlação entre peroxidação lipídica e o perfil de marcadores inflamatórios em pacientes portadores de diabetes mellitus tipo 2 com periodontite crônica
Beneficiário:Silvana Regina Perez Orrico
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/16148-0 - Investigação do transcriptoma e do proteoma salivar e plasmático em indivíduos acometidos por diabetes mellitus tipo 2, dislipidemia e doença periodontal crônica
Beneficiário:Sâmia Cruz Tfaile Corbi
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 10/10882-2 - Expressão gênica em indivíduos portadores de Diabetes mellitus tipo 2, dislipidemia e doença periodontal: avaliação por microarray
Beneficiário:Raquel Mantuaneli Scarel Caminaga
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/16233-9 - Avaliação do efeito da descompensação do Diabetes Mellitus sobre a expressão de genes do sistema imune e sobre lesões no DNA em pacientes com periodontite crônica.
Beneficiário:Sâmia Cruz Tfaile Corbi
Modalidade de apoio: Bolsas no Brasil - Doutorado