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Effects of Contemporary Irrigant Activation Schemes and Subsequent Placement of an Interim Dressing on Bacterial Presence and Activity in Root Canals Associated with Asymptomatic Apical Periodontitis

Texto completo
Carvalho, Alexandre P. L. [1] ; Nardello, Laura C. L. [1] ; Fernandes, Fernanda S. [1] ; Bruno, Fernanda P. [1] ; Paz, Luiza R. [1] ; Iglecias, Elaine F. [1] ; Honorio, Heitor M. [2] ; Mayer, Marcia P. A. [3] ; Gavini, Giulio [1] ; Pinheiro, Ericka T. [1, 4]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Dent, Dept Dent, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, BR-17012901 Bauru, SP - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508000 Sao Paulo - Brazil
[4] Univ Zurich, Ctr Dent Med, Clin Conservat & Prevent Dent, CH-8032 Zurich - Switzerland
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Citações Web of Science: 0

New tools for activating endodontic irrigants have evolved, yet their impact on root canal disinfection, in comparison to the passive placing of an inter-visit medication, have not yet been fully elucidated. The use of DNA- and rRNA-based methods may cast some new light on this issue, as they allow a comparison to be made between microbial presence and activity. Therefore, the aim of this single-arm intervention trial is to evaluate the antibacterial effect of endodontic procedures using both molecular methods. Root canal samples were obtained from 20 patients with asymptomatic apical periodontitis after each treatment step: access cavity, chemo-mechanical preparation, adjunctive procedures (XP-endo Finisher file and passive ultrasonic irrigation), calcium hydroxide medication, and 2nd-visit root canal preparation. DNA and cDNA from the samples were subjected to quantitative polymerase chain reaction with universal primers for the bacterial 16S rRNA gene. Chemo-mechanical preparation promoted a drastic reduction in bacterial levels and activity, whereas the adjunctive procedures did not make a significant contribution to further disinfection. At the 2nd visit, bacteria were active after the use of calcium hydroxide medication; however, they were significantly reduced after a 2nd-visit preparation. Consequently, the lowest bacterial levels were found at the end of the treatment. This clinical trial, which used an rRNA and rDNA combined approach, confirmed previous studies showing that root canal preparation represents the main strategy for root canal disinfection. (AU)

Processo FAPESP: 16/15473-0 - Análise da Comunidade Bacteriana Metabolicamente Ativa nas Infecções Endodônticas Persistentes após Diferentes Protocolos Clínicos de Desinfecção: Estudo Molecular baseado em RNA e DNA
Beneficiário:Ericka Tavares Pinheiro
Modalidade de apoio: Auxílio à Pesquisa - Regular