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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Human intestinal Caco-2 cell line in vitro assay to evaluate the absorption of Cd, Cu, Mn and Zn from urban environmental matrices

Texto completo
Boim, Alexys Giorgia Friol [1] ; Wragg, Joanna [2] ; Canniatti-Brazaca, Solange Guidolin [3] ; Alleoni, Luis Reynaldo Ferracciu [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Luiz Queiroz Coll Agr, Dept Soil Sci, ESALQ, USP, BR-13418-900 Piracicaba, SP - Brazil
[2] Environm Sci Centre, British Geological Survey, Nicker Hill, Keyworth, Nottingham - England
[3] Dept Agri, Food Ind, Food, Nutr, ESALQ, USP, Piracicaba - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: ENVIRONMENTAL GEOCHEMISTRY AND HEALTH; v. 42, n. 2, p. 601-615, FEB 2020.
Citações Web of Science: 3

The Caco-2 cell line is derived from a human colon adenocarcinoma and is generally used in toxicity assays. The ingestion of soil or dust is a significant route of human exposure to potential harmful elements (PHE), and assays of bioaccessibility or bioavailability can be used to measure the potential hazard posed by exposure to toxic substances. The in vitro digestion (UBM method) and Caco-2 cell model were used to investigate the bioaccessibility and absorption by intestinal cells of the PHE in four matrices (two urban soils and two soils with lead (Pb)-mining tailings) along with the guidance material for bioaccessibility measurements, BGS 102. The gastrointestinal (GI) compartment was simulated, and the resulting material added to Caco-2 cells. In the GI, the average bioaccessibility was 24% for cadmium (Cd), 17% for copper (Cu), 0.2% for Pb, 44% for manganese (Mn) and 6% for zinc (Zn). The poor reproducibility was attributed to the pH (6.3) and the highly complex GI fluid that formed PHE precipitates and complexes. In 2 h, Caco-2 cells absorbed 0.2 ng mg(-1) of cellular protein for Cd, 13.4 ng mg(-1) for Cu, 5 ng mg(-1) for Mn and 31.7 mu g mg(-1) for Zn. Lead absorption was lower than the limit of quantification (< 2 mu g L-1). Cd was presented in the cell monolayer and could interfere in the intracellular accumulation of Cu, Mn and Zn. The use of in vitro assays allowed for an estimation of the absorption of Cd, Cu, Mn and Zn from environmental matrices to be made, and except for Mn, it had a positive correlation with bioaccessible concentration, suggesting a common association of these elements in the cellular environment. (AU)

Processo FAPESP: 15/19332-9 - Bioacessibilidade oral e respiratória de elementos potencialmente tóxicos no solo e em sedimentos urbanos
Beneficiário:Alexys Giorgia Friol Boim
Modalidade de apoio: Bolsas no Brasil - Doutorado