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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Unveiling resistome profiles in the sediments of an Antarctic volcanic island

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Autor(es):
Centurion, V. B. [1, 2] ; Delforno, T. P. [1] ; Lacerda-Junior, G. V. [1, 3] ; Duarte, A. W. F. [1, 4] ; Silva, U. [3] ; Bellini, G. B. [1, 2] ; Rosa, L. H. [5] ; Oliveira, V. M. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] State Univ Campinas UNICAMP, Res Ctr Chem Biol & Agr CPQBA, Microbial Resources Div, BR-13081970 Paulinia, SP - Brazil
[2] State Univ Campinas UNICAMP, Biol Inst, BR-13083862 Campinas, SP - Brazil
[3] Brazilian Agr Res Corp EMBRAPA, BR-13820000 Jaguariuna, SP - Brazil
[4] Univ Fed Alagoas, Campus Arapiraca UFAL, BR-57309005 Arapiraca, AL - Brazil
[5] Fed Univ Minas Gerais UFMG, Inst Biol Sci, BR-31270901 Belo Horizonte, MG - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Environmental Pollution; v. 255, n. 2 DEC 2019.
Citações Web of Science: 0
Resumo

The Deception Island, located in Maritime Antarctica, is a volcanic island with geothermal activity and one of the most visited by tourists. However, the extent of the anthropogenic impact remains largely unknown and the factors shaping the resistance/tolerance mechanisms in the microbiomes from Whalers Bay ecosystems have never been investigated. In this context, this study aimed to reveal the resistome profiles of Whalers Bay sediments and correlate them with environmental factors. Samples were collected at four sites during the summer 2014/2015 along a transect of 27.5 m in the Whalers Bay sediments. DNA isolated from sediment samples was sequenced using the Illumina HiSeq platform. Bioinformatic analyses allowed the assembly of contigs and scaffolds, prediction of ORFs, and taxonomic and functional annotation using NCBI RefSeq database and KEGG orthology, respectively. Microorganisms belonging to the genera Psychrobacter, Flavobacterium and Polaromonas were shown to dominate all sites, representing 60% of taxonomic annotation. Arsenic (As), copper (Cu) and iron (Fe) were the most abundant metal resistance/tolerance types found in the microbiomes. Beta-lactam was the most common class related to antibiotics resistance/tolerance, corroborating with previous environmental resistome studies. The acridine class was the most abundant amongst the biocide resistance/tolerances, related to antiseptic compounds. Results gathered in this study reveal a repertoire of resistance/tolerance classes to antibiotics and biocides unusually found in Antarctica. However, given the volcanic nature (heavy metals-rich region) of Deception Island soils, this putative impact must be viewed with caution. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 16/05640-6 - Multi-ômicas aplicadas ao conhecimento e exploração de microbiomas Antárticos
Beneficiário:Valeria Maia Merzel
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/03172-8 - Mecanismos de adaptação ao estresse em microbioma de sedimentos da Ilha Vulcânica Deception na Antártica marítima
Beneficiário:Victor Borin Centurion Biruel
Linha de fomento: Bolsas no Brasil - Doutorado Direto