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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Saccharification of pretreated sugarcane bagasse using enzymes solution from Pycnoporus sanguineus MCA 16 and cellulosic ethanol production

Texto completo
Autor(es):
Pereira Scarpa, Josiani de Cassia [1] ; Marques, Natalia Paganini [2] ; Monteiro, Diego Alves [1] ; Martins, Gisele Marta [1] ; de Paula, Ariela Veloso [3] ; Boscolo, Mauricio [1] ; da Silva, Roberto [1] ; Gomes, Eleni [1] ; Bocchini, Daniela Alonso [2]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Inst Biosci Letters & Exact Sci, Sao Jose Do Rio Preto, SP - Brazil
[2] Sao Paulo State Univ UNESP, Inst Chem, Araraquara, SP - Brazil
[3] Sao Paulo State Univ UNESP, Fac Pharmaceut Sci, Araraquara, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: INDUSTRIAL CROPS AND PRODUCTS; v. 141, DEC 1 2019.
Citações Web of Science: 0
Resumo

Pycnoporus swsguineus MCA 16 was cultivated by solid state fermentation on lignocellulosic residues, at 40 degrees C. The highest endoglucanase, exoglucanase, beta-glucosidase, xylanase, beta-xylosidase and laccase activities (200, 29.1, 52.1, 44.2, 1.81 and 22.04 U g(-1), respectively) were obtained when using the mixture of wheat bran and soybean meal (1:1 w/w) as substrates, at 96 h of cultivation. Peroxidases highest activities were observed at 48 h (14.61 and 10.02 U g(-1) for manganese and lignin peroxidase, respectively). Enzymatic saccharification of sugarcane bagasse submitted to hydrothermal alkaline pretreatment provided glucose at 7.32 g L-1 when using solid load of 13.5% (dry basis), 288.3 total units of endoglucanase/g of cellulose, 130 h and 57 degrees C. Ligninases present in the enzymatic solution provided a reduction of 82.3% in the concentration of total phenolic compounds during saccharification. The hydrolysate was used for ethanol production by Saccharomyces cerevisiae CAT-1 and a yield of 73.5% was obtained. (AU)

Processo FAPESP: 11/19554-0 - Prospecção de fungos filamentosos termofílicos produtores de celulases e aplicação das enzimas na sacarificação do bagaço de cana-de-açúcar
Beneficiário:Daniela Alonso Bocchini
Modalidade de apoio: Auxílio à Pesquisa - Regular