Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Semi-quantitative analysis of morphological changes in bee tissues: A toxicological approach

Texto completo
Autor(es):
Grella, Tatiane Caroline [1, 2] ; Soares-Lima, Hellen Maria [3] ; Malaspina, Osmar [2] ; Ferreira Nocelli, Roberta Cornelio [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Ctr Ciencias Agr, Dept Ciencias Nat Matemat & Educ, Campus Araras, Rodovia Anhanguera SP-330, Km 174, BR-13600970 Araras, SP - Brazil
[2] Univ Estadual Paulista, Dept Biol, Ctr Estudos Insetos Socials, Ave 24-A, 1-515, BR-13506900 Rio Claro, SP - Brazil
[3] Univ Fed Sao Carlos, Dept Biol, Ctr Ciencias & Tecnol Sustentabilidade, Campus Sorocaba, Rodovia Joao Leme dos Santos, BR-18052780 Sorocaba, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Chemosphere; v. 236, DEC 2019.
Citações Web of Science: 2
Resumo

During foraging, bees are exposed to sublethal doses of insecticides, which can cause morphological changes to various organs, such as the midgut, Malpighian tubules, and mushroon body. Thus, the purpose of this study was to establish a scoring system to evaluate these alterations based on the damage caused and its reversibility. Therefore, a grade 1 score indicates a minimal and easily reversible lesion, increased apocrine secretion, increased cell elimination into the lumen, and a larger quantity of spherocrystals; grade 2 was assigned to moderate and typically reversible injuries, such as changes in the brush border, vacuolation/loss of cytoplasmic material, presence/height of the brush border, and cell swelling; and grade 3 was assigned to serious and irreversible, loss of cell nests of regenerative cells, pyknosis, and loss of contact between Kenyon cells. In addition, frequency values were assigned since the alterations can occur at different frequencies according to the insecticide and the bees exposed; the frequency ranges from 0 to 6, with 0 representing the absence of an alteration and 6 representing a high frequency occurrence. Based on the analyses, we conclude that each change causes morphological damage, which may or may not be irreversible and could affect the health of the colony. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 14/12488-0 - Avaliação de risco dos agrotóxicos sobre abelhas e seus impactos sobre o processo de polinização
Beneficiário:Roberta Cornélio Ferreira Nocelli
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/09555-5 - Efeitos sinergísticos entre Nosema sp. e o inseticida imidacloprido sobre abelhas Apis mellifera africanizadas
Beneficiário:Hellen Maria Soares Lima
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 17/21097-3 - Interações abelha-agricultura: perspectivas para a utilização sustentável
Beneficiário:Osmar Malaspina
Linha de fomento: Auxílio à Pesquisa - Temático