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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Recruitment of monocytes and mature macrophages in mouse pubic symphysis relaxation during pregnancy and postpartum recovery

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Autor(es):
Castelucci, Bianca G. [1] ; Consonni, Silvio R. [1] ; Rosa, Viviane S. [1] ; Joazeiro, Paulo P. [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: BIOLOGY OF REPRODUCTION; v. 101, n. 2, p. 466-477, AUG 2019.
Citações Web of Science: 0
Resumo

Appropriate remodeling of the female lower reproductive tract and pelvic floor is essential during normal mammalian pregnancy, labor, and postpartum recovery. During mouse pregnancy, in addition to reproductive tract modifications, the pubic symphysis (PS) is remodeled into a soft interpubic ligament (IpL) to provide safe delivery of the offspring and fast postpartum recovery. Although temporal changes in the phenotypes of myeloid cells, such as mononuclear phagocytes, are crucial to remodeling the lower reproductive tract organs in preparation for a safe delivery, little is known about the involvement of recruited monocytes or macrophages inmouse PS remodeling. We used combined light microscopy, electron microscopy, and qPCR analysis to investigate the profile of recruited monocytes and macrophage polarization markers in C57Bl6 mouse interpubic tissues during pregnancy (D12, D18, and D19) and early days postpartum (1 dpp and 3 dpp) to better identify their presence in proper remodeling of the mouse PS. Our morphological data show that the number of recruited monocytes is increased in interpubic tissues and that recruited monocytes differentiate into proinflammatory or anti-inflammatory macrophage phenotypes from D18 to 3 dpp, which may contribute to dynamic changes in the gene expression of specific inflammatory mediators involved in interpubic tissue remodeling at these time points. Therefore, our morphological and quantitative gene expression data suggest that both differentiated macrophages from recruited monocytes and polarized macrophages may collaborate for IpL relaxation at labor and the appropriate repair of the PS after the first pregnancy. Summary Sentence Recruited monocytes and mature macrophages are present in the mouse pubic symphysis and may contribute to mouse pubic symphysis relaxation during late pregnancy and postpartum recovery. (AU)

Processo FAPESP: 12/25038-8 - Influencia da Depleção de Macrófagos na Remodelação da Sínfise Púbica de camundongos C57BL6, Durante o final da Prenhês e o Pós-parto.
Beneficiário:Bianca Gazieri Castelucci
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 15/23616-2 - Caracterização de estados de ativação de monócitos e macrófagos presentes no remodelamento da sínfise púbica do camundongo, durante o final da prenhez e o pós-parto.
Beneficiário:Paulo Pinto Joazeiro
Linha de fomento: Auxílio à Pesquisa - Regular