Mafra, Fernando F. P.
Macedo, Michel M.
Orphao, Juliana do N. P.
Lopes, Arthur Vecchi
Teixeira, Carla de Brito
Gattai, Pedro P.
Nascimento, Fabio Dupart
Brandao Lopes-Martins, Rodrigo Alvaro
Número total de Autores: 9
Afiliação do(s) autor(es):
 Univ Mogi das Cruzes, Technol Res Ctr, Mogi das Cruzes - Brazil
 Fed Univ Sa Paulo UNIFESP, Mol Biol Lab, Div Renal, Dept Med, Mol - Brazil
 Univ Vale Paraiba Univ, Inst Res & Dev, Ave Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil
Número total de Afiliações: 3
Tipo de documento:
PHOTOBIOMODULATION PHOTOMEDICINE AND LASER SURGERY;
Citações Web of Science:
Background: The lipid metabolism is essential for maintaining the body's energy responses. Laser photobiomodulation triggers many important cellular effects, but these effects on lipid metabolism are not well described. In this study, we analyzed the laser photobiomodulation in the hormone-sensitive lipase (HSL) activity, a key enzyme in the triglycerides (TAG) hydrolysis in adipose tissue 3T3-L1. Methods: Cells were submitted to the differentiation protocol in adipose cells, irradiated with 1, 2, and 3J with laser (904 nm-60mw-laser diode) and incubated for 4 h after irradiation. Results: The response of laser photobiomodulation was able to trigger an inhibition of HSL activity (control = 0.057 - 0.0008; 1J = 0.050 +/- 0.0003; 2J = 0.0477 +/- 0.002; 3J = 0.051 +/- 0.002; p = 0.0003 against the control), but no modulation was observed in TAG levels into the medium (control = 26.5856 +/- 0.52; 1J = 26.5856 +/- 0.52; 2J = 27.2372 +/- 1.41; 3J = 25.9991 +/- 0.1303; p = 0.18). Conclusions: This is the first study of HSL activity modulation with laser radiation, suggesting that photobiomodulation can influence adipose tissue metabolism and open a new field of study. (AU)