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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Laser Photobiomodulation 904nm Promotes Inhibition of Hormone-Sensitive Lipase Activity in 3T3-L1 Adipocytes Differentiated Cells

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Autor(es):
Mafra, Fernando F. P. [1] ; Macedo, Michel M. [1] ; Orphao, Juliana do N. P. [1] ; Lopes, Arthur Vecchi [1] ; Teixeira, Carla de Brito [1] ; Gattai, Pedro P. [2] ; Torres-Silva, Romildo [1] ; Nascimento, Fabio Dupart [1] ; Brandao Lopes-Martins, Rodrigo Alvaro [3]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Mogi das Cruzes, Technol Res Ctr, Mogi das Cruzes - Brazil
[2] Fed Univ Sa Paulo UNIFESP, Mol Biol Lab, Div Renal, Dept Med, Mol - Brazil
[3] Univ Vale Paraiba Univ, Inst Res & Dev, Ave Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: PHOTOBIOMODULATION PHOTOMEDICINE AND LASER SURGERY; v. 37, n. 2, p. 66-69, FEB 2019.
Citações Web of Science: 0
Resumo

Background: The lipid metabolism is essential for maintaining the body's energy responses. Laser photobiomodulation triggers many important cellular effects, but these effects on lipid metabolism are not well described. In this study, we analyzed the laser photobiomodulation in the hormone-sensitive lipase (HSL) activity, a key enzyme in the triglycerides (TAG) hydrolysis in adipose tissue 3T3-L1. Methods: Cells were submitted to the differentiation protocol in adipose cells, irradiated with 1, 2, and 3J with laser (904 nm-60mw-laser diode) and incubated for 4 h after irradiation. Results: The response of laser photobiomodulation was able to trigger an inhibition of HSL activity (control = 0.057 - 0.0008; 1J = 0.050 +/- 0.0003; 2J = 0.0477 +/- 0.002; 3J = 0.051 +/- 0.002; p = 0.0003 against the control), but no modulation was observed in TAG levels into the medium (control = 26.5856 +/- 0.52; 1J = 26.5856 +/- 0.52; 2J = 27.2372 +/- 1.41; 3J = 25.9991 +/- 0.1303; p = 0.18). Conclusions: This is the first study of HSL activity modulation with laser radiation, suggesting that photobiomodulation can influence adipose tissue metabolism and open a new field of study. (AU)

Processo FAPESP: 15/06502-3 - Análise e Modelagem do Comportamento Mecânico de Tendões Lesionados Mecanicamente e Submetidos ao Tratamento com Laserterapia de Baixa Potência e Crioterapia
Beneficiário:Rodrigo Alvaro Brandão Lopes Martins
Modalidade de apoio: Auxílio à Pesquisa - Regular