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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Efficiency of CellROX deep red (R) and CellROX orange (R) fluorescent probes in identifying reactive oxygen species in sperm samples from high and low fertility bulls

Texto completo
Autor(es):
Carvalho Celeghini, Eneiva Carla [1] ; Rodrigues Alves, Maira Bianchi [1] ; de Arruda, Rubens Paes [1] ; de Rezende, Gabriela Marques [1] ; Florez-Rodriguez, Shirley Andrea [1] ; de Sa Filho, Manoel Francisco [1, 2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, St Duque Caxias Norte 225, BR-13635900 Pirassununga - Brazil
[2] Alta Genet, Uberaba - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: ANIMAL BIOTECHNOLOGY; v. 32, n. 1 AUG 2019.
Citações Web of Science: 10
Resumo

Detection of reactive oxygen species (ROS) is of great interest in semen analysis since their excess is detrimental to sperm function and male fertility. Fluorescence microscopy has achieved attention for providing broad possibilities of sperm evaluations and also for presenting substantial accessibility. In this context, this study investigated the efficiency of CellROX Deep Red (R) and Orange (R) probes in detecting ROS in bovine sperm cells and assessed their relationship with sperm fertility potential. First, 16 ejaculates were assigned in three treatments: T0 (no ROS production induced), T1x (ROS production induced once) and T2x (ROS production induced twice). Samples were incubated with Red and Orange probes and percentages of cells producing ROS were evaluated using fluorescence microscopy. Coefficient of determination was 0.61 for Red and 0.56 for Orange. Afterwards, frozen-thawed semen samples from high and low fertility bulls were evaluated regarding percentages of cells producing ROS detected by Red and Orange. Higher levels of ROS assessed by Red were detected in low fertility bovine samples. In conclusion, CellROX Red (R) and Orange (R) are both efficient in detecting ROS in bovine spermatozoa. Furthermore, higher sperm ROS detection by CellROX Red (R) might be associated with low fertility samples. (AU)

Processo FAPESP: 15/09154-6 - Perfil de microRNAs presentes no sêmen e embriões bovinos e sua relação com a fertilidade
Beneficiário:Maíra Bianchi Rodrigues Alves
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 16/05395-1 - Perfil de microRNAs presentes no sêmen e embriões bovinos e sua relação com a fertilidade
Beneficiário:Eneiva Carla Carvalho Celeghini
Modalidade de apoio: Auxílio à Pesquisa - Regular