Henrique, Maressa O.
Neto, Leila S.
Assis, Josiane B.
Barros, Michele S.
Capurro, Margareth L.
Lepique, Ana P.
Fonseca, Denise M.
Número total de Autores: 8
Afiliação do(s) autor(es):
 Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, Lab Imunol Expt, BR-05508900 Sao Paulo, SP - Brazil
 Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, Lab Mosquitos Geneticamente Modificados, Sao Paulo, SP - Brazil
 Conselho Nacl Desenvolvimento Cient & Tecnol INCT, Inst Nacl Ciencia & Tecnol Entomol Mol, Rio De Janeiro, RJ - Brazil
 Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, Lab Imunomodulacao, Sao Paulo, SP - Brazil
 Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, Lab Imunol Mucosas, BR-05508900 Sao Paulo, SP - Brazil
Número total de Afiliações: 5
Tipo de documento:
Citações Web of Science:
During probing and blood feeding, haematophagous mosquitoes inoculate a mixture of salivary molecules into their vertebrate hosts' skin. In addition to the anti-haemostatic and immunomodulatory activities, mosquito saliva also triggers acute inflammatory reactions, especially in sensitized hosts. Here, we characterize the oedema and the cellular infiltrate following Aedes aegypti mosquito bites in the skin of sensitized and non-sensitized BALB/c mice by flow cytometry. Ae. aegypti bites induced an increased oedema in the ears of both non-sensitized and salivary gland extract- (SGE-)sensitized mice, peaking at 6 hr and 24 hr after exposure, respectively. The quantification of the total cell number in the ears revealed that the cellular recruitment was more robust in SGE-sensitized mice than in non-sensitized mice, and the histological evaluation confirmed these findings. The immunophenotyping performed by flow cytometry revealed that mosquito bites were able to produce complex changes in cell populations present in the ears of non-sensitized and SGE-sensitized mice. When compared with steady-state ears, the leucocyte populations significantly recruited to the skin after mosquito bites in non-sensitized and sensitized mice were eosinophils, neutrophils, monocytes, inflammatory monocytes, mast cells, B-cells and CD4(+) T-cells, each one with its specific kinetics. The changes in the absolute number of cells suggested two cell recruitment profiles: (i) a saliva-dependent migration; and (ii) a migration dependent on the immune status of the host. These findings suggest that mosquito bites influence the skin microenvironment by inducing differential cell migration, which is dependent on the degree of host sensitization to salivary molecules. (AU)