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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Improved antibody loading on self-assembled graphene oxide films for using in surface plasmon resonance immunosensors

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Autor(es):
Miyazaki, Celina Massumi [1] ; Camilo, Douglas Eleuterio [1] ; Shimizu, Flavio Makoto [2, 3] ; Ferreira, Marystela [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, CCTS, Rd Joao Leme Santos, Km 110, BR-18052780 Sorocaba, SP - Brazil
[2] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab LNNano, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Applied Surface Science; v. 490, p. 502-509, OCT 1 2019.
Citações Web of Science: 0
Resumo

Immunoassays are important for the diagnosis of many diseases. Efficient methods for the immobilization of the biorecognition elements is a relevant subject because the antibody loading is directly related to the detection range and sensitivity. Here different self-assembled films were explored to take advantage of polar oxygenated groups from graphene oxide (GO) structure. Surface plasmon resonance (SPR) sensors were modified with different GO self-assembled films and compared to the well-established thiol-functionalization with 11-MUA (11-mercaptoundecanoic acid). Self-assembled cysteamine/GO functionalized sensor (Cys-GO(SA)) provided the highest protein loading (7.66 x 10(-12) mol cm(-2) against 1.29 x 10(-11) mol cm(-2) for the traditional thiolfunctionalization). The Langmuir-Blodgett (LB) films of GO were composed of aggregates and empty spaces, which hindered the antibody anchoring (around 6.38 x 10(-12) to 3.45 x 10(-12) mol cm(-2)). The SPR response of the Cys-GO(SA)-modified sensor to the 4.0 ng mL(-1) PSA was 125% higher than the thiol-functionalized sensor at the same concentration. This result indicates a simple and promising surface modification strategy for many important applications, not limited to SPR sensors, but for all immunoreaction-based assays as enzyme-linked immunosorbent assay (ELISA), electrochemical (e.g. amperometric and impedimetric) and quartz crystal microbalance (QCM) based sensors. (AU)

Processo FAPESP: 14/15093-7 - Estudos de sistemas nanoestruturados por ressonância plasmônica de superfície (SPR): aplicações em biossensoriamento
Beneficiário:Celina Massumi Miyazaki
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 16/19387-0 - Desenvolvimento de filmes nanoestruturados automontados (layer-by-layer) e langmuir-blodgett (lb) incorporando emodina para sistemas carreadores
Beneficiário:Marystela Ferreira
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/50869-6 - INCT 2014: em Eletrônica Orgânica INEO
Beneficiário:Roberto Mendonça Faria
Modalidade de apoio: Auxílio à Pesquisa - Temático