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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Molecular discrimination of Ancistrus lineages (Siluriformes: Loricariidae) using barcode DNA tool

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Autor(es):
de Borba, Rafael Splendore [1] ; Mariotto, Sandra [2] ; Centofante, Liano [3] ; Zawadzki, Claudio Henrique [4] ; Parise-Maltempi, Patricia Pasquali [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ Unesp, Inst Biosci, Rio Claro - Brazil
[2] Mato Grosso Fed Inst IFMT, Cuiaba - Brazil
[3] Univ Fed Mato Grosso, Inst Biosci, Cuiaba - Brazil
[4] Univ Estadual Maringa, Dept Biol, Ctr Res Limnol Ichthyol & Aquaculture Nupelia, Maringa, Parana - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: MITOCHONDRIAL DNA PART A; v. 30, n. 4 APR 2019.
Citações Web of Science: 0
Resumo

Although several species of Ancistrus have been described from the Amazon and Paraguay river basins in the states of Amazonas and Mato Grosso, Brazil, the taxonomic status of most specimens from these regions remains doubtful. In the present work, cytogenetic and molecular data were used to discriminate and isolate unexpected Ancistrus lineages from the Amazon and Paraguay basins. For that, it was used DNA barcoding based on mitochondrial Cytochrome Oxidase Subunit I (COI) gene and cytogenic data to perform such molecular discrimination. The analyzed sequences had 669 bp, of which 171 bp were conserved and 491 bp were variable. The Neighbor-joining and Bayesian analysis revealed 21 distinct groups in topology. The genetic distances within each group was 0.4%, 21 times smaller than the mean distance observed among groups, which was 8.4%. These values showed seven distinct lineages of Ancistrus from the studied points of the Amazon basin and eight lineages from the Paraguay basin points. Our results illustrate the efficiency of this technique for the discrimination of the Ancistrus lineages once it indicates the occurrence of cryptic species in these regions, which cannot yet be identified either with just chromosomal or morphological analyzes. (AU)

Processo FAPESP: 13/17211-4 - Estudo dos cromossomos e de filogenia molecular de peixes do gênero Ancistrus (Siluriformes, Loricariidae)
Beneficiário:Patricia Pasquali Parise Maltempi
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/17826-9 - Relações filogenéticas, filogeográficas e discriminação molecular de espécies do gênero ancistrus kner, 1854 (siluriformes: loricariidae) que compõem três bacias hidrográficas do estado do mato grosso, brasil.
Beneficiário:Rafael Splendore de Borba
Linha de fomento: Bolsas no Brasil - Doutorado