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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Improvements in metal exposure assays: artificial food to assess bioaccumulation in the blue crab Callinectes danae Smith, 1869 (Crustacea, Decapoda, Portunidae)

Texto completo
Autor(es):
Bordon, Isabella C. [1] ; de Campos, Bruno Galvao [1] ; Gusso-Choueri, Paloma Kachel [1] ; Miyai, Caio Akira [2] ; de Araujo, Giuliana Seraphim [1] ; Emerenciano, Andrews Krupinski [3] ; Machado Cunha da Silva, Jose Roberto [3] ; Barboza Cotrim, Marycel Elena [4] ; Teixeira Favaro, Deborah Ines [5] ; de Souza Abessa, Denis Moledo [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, NEPEA, Campus Litoral Paulista, BR-11330900 Sao Vicente, SP - Brazil
[2] UNESP Univ Estadual Paulista, Lab Ecol & Comportamento Anim Labecom, Campus Litoral Paulista, BR-11330900 Sao Vicente, SP - Brazil
[3] Univ Sao Paulo, Biomed & Biol Sci Inst ICB, Av Prof Lineu Prestes 1524, BR-05508900 Sao Paulo, SP - Brazil
[4] IPEN Nucl & Energy Res Inst, Chem & Environm Technol Ctr, Ave Prof Lineu Prestes 2242, BR-05508000 Sao Paulo, SP - Brazil
[5] IPEN Nucl & Energy Res Inst, Res Reactor Ctr, Ave Prof Lineu Prestes 2242, BR-05508000 Sao Paulo, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: International Journal of Environmental Research; v. 13, n. 2, p. 431-434, APR 2019.
Citações Web of Science: 0
Resumo

This technical note aims to report, in detail, the development, suitability and analytical validation of an artificial food to assess metal bioaccumulation in the blue crab Callinectes danae after metal exposure assays. For this purpose, lead (Pb) was chosen as the metal of interest. First, a non-contaminated artificial food was prepared by mixing commercial shrimps and agarose. The food was then offered on alternate days to two groups of crabs for 7 and 14days, respectively. After confirming that this food was well-accepted by crabs, analytical procedures were performed to confirm that Pb added to the mixture would remain in the artificial food. For this purpose, two foods containing Pb at concentrations of 0.5 and 2.0 mu g g(-1) were prepared by adding aliquots of an ultrapure Pb standard solution to the mixture. Samples of each food were subjected to an acid extraction and Pb concentrations were determined by a GF-AAS. Pb recoveries in both contaminated foods were > 90%. Subsequently, another experiment was performed, and two groups of crabs were fed with contaminated artificial foods (0.5 and 2.0 mu g g(-1) of Pb) during 7 and 14days, respectively. The observed acceptance of these foods by crabs at both Pb concentrations was of 100%. Article HighlightsAn artificial food was prepared to assess bioaccumulation in the blue crabLead (Pb) was chosen as the metal of interestThe acceptance of artificial food was evaluatedIn the contaminated artificial foods, Pb was determinedIt was concluded that this food can be applied in metal exposure assays (AU)

Processo FAPESP: 14/01576-6 - Análise dos efeitos da exposição ao Pb por via alimentar e respiratória no siri azul Callinectes danae
Beneficiário:Isabella Cristina da Costa Leal Bordon
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado