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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Nanosynthesis of Silver-Calcium Glycerophosphate: Promising Association against Oral Pathogens

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Autor(es):
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Fernandes, Gabriela Lopes [1] ; Botazzo Delbem, Alberto Carlos [2] ; do Amaral, Jackeline Gallo [2] ; Gorup, Luiz Fernando [3, 4] ; Fernandes, Renan Aparecido [5, 1] ; de Souza Neto, Francisco Nunes [3] ; Santos Souza, Jose Antonio [2] ; Monteiro, Douglas Roberto [6] ; Agostinho Hunt, Alessandra Marcal [7] ; Camargo, Emerson Rodrigues [3] ; Barbosa, Debora Barros [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, BR-16015050 Sao Paulo - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Pediat Dent & Publ Hlth, BR-16015050 Sao Paulo - Brazil
[3] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Paulo - Brazil
[4] Fed Univ Grande Dourados, Dept Chem, FACET, BR-79804970 Dourados, MS - Brazil
[5] Univ Ctr Adamantina UNIFAI, Dept Dent, BR-17800000 Sao Paulo - Brazil
[6] Univ Western Sao Paulo UNOESTE, Grad Program Dent, BR-19050920 Sao Paulo - Brazil
[7] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48823 - USA
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: ANTIBIOTICS-BASEL; v. 7, n. 3 SEP 2018.
Citações Web of Science: 3
Resumo

Nanobiomaterials combining remineralization and antimicrobial abilities would bring important benefits to control dental caries. This study aimed to produce nanocompounds containing calcium glycerophosphate (CaGP) and silver nanoparticles (AgNP) by varying the reducing agent of silver nitrate (sodium borohydride (B) or sodium citrate (C)), the concentration of silver (1% or 10%), and the CaGP forms (nano or commercial), and analyze its characterization and antimicrobial activity against ATCC Candida albicans (10231) and Streptococcus mutans (25175) by the microdilution method. Controls of AgNP were produced and silver ions (Ag+) were quantified in all of the samples. X-ray diffraction, UV-Vis, and scanning electron microscopy (SEM) analysis demonstrated AgNP associated with CaGP. Ag+ ions were considerably higher in AgCaGP/C. C. albicans was susceptible to nanocompounds produced with both reducing agents, regardless of Ag concentration and CaGP form, being Ag10%CaGP-N/C the most effective compound (19.5-39.0 mu g Ag mL(-1)). While for S. mutans, the effectiveness was observed only for AgCaGP reduced by citrate, also presenting Ag10%CaGP-N the highest effectiveness (156.2-312.5 mu g Ag mL(-1)). Notably, CaGP enhanced the silver antimicrobial potential in about two- and eight-fold against C. albicans and S. mutans when compared with the AgNP controls (from 7.8 to 3.9 and from 250 to 31.2 mu g Ag mL(-1), respectively). The synthesis that was used in this study promoted the formation of AgNP associated with CaGP, and although the use of sodium borohydride (B) resulted in a pronounced reduction of Ag+, the composite AgCaGP/B was less effective against the microorganisms that were tested. (AU)

Processo FAPESP: 14/08648-2 - Nanocompósito prata/²-glicerofosfato de cálcio: síntese, efeito sobre biofilmes de Candida albicans e Streptococcus mutans e sobre células odontoblásticas
Beneficiário:Gabriela Lopes Fernandes
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 13/24200-9 - Efeito do nanocompósito prata/B-glicerofosfato de cálcio sobre biofilmes de Candida albicans e Streptococcus mutans e sobre células de polpa dental humana na presença e ausência desses biofilmes
Beneficiário:Debora Barros Barbosa
Linha de fomento: Auxílio à Pesquisa - Regular