Experimental inoculation of gilts with bovine vira... - BV FAPESP
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Experimental inoculation of gilts with bovine viral diarrhea virus 2 (BVDV-2) does not induce transplacental infection

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Autor(es):
Pereira, Daniele Araujo [1] ; Peron, Juliana Brigolin [1] ; de Souza Almeida, Henrique Meiroz [1] ; Baraldi, Thais Gasparini [1] ; Honorato Gatto, Igor Renan [1] ; Kasmanas, Thaiane Coelho [1] ; Pituco, Edviges Maristela [2] ; Montassier, Helio Jose [1] ; de Oliveira, Luis Guilherme [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ Unesp, Sch Agr & Vet Sci FCAV, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil
[2] Biol Inst Sao Paulo, Av Conselheiro Rodrigues Alves 1252, BR-04014002 Sao Paulo, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Veterinary Microbiology; v. 225, p. 25-30, NOV 2018.
Citações Web of Science: 1
Resumo

Bovine viral diarrhea virus (BVDV) belongs to the genus Pestivirus and can cause reproductive problems in cattle. However, there is still a lack of research to clarify its pathogenicity in different gestational periods of sows and its effects in neonates. In this study, 12 gilts divided into groups (G) were experimentally inoculated with the strain BVDV-2 (SV-253) oronasally at a dose of 10(6.85) TCID50; one group was inoculated 30 days before insemination (GO; n = 2), three groups were inoculated during gestation (first (G1; n = 2), second (G2; n = 3), third (G3; n = 3)), and a fourth was the control group (G4; n = 2). Samples of blood and nasal swabs from the gilts were collected every three days until delivery for a virus neutralization (VN) test, qRT-PCR, and blood count. On the day of delivery, 40% of the neonates were euthanized to obtain tissue and blood samples at necropsy for histopathology and qRT-PCR. The sows were seroconverted between 12 and 33 days after inoculation, and the virus was detected in the blood between 3 and 12 days and on the nasal swab between 6 and 24 days in the G0, G1, G2 and G3 sows but was not detected in piglet tissues, and no significant alterations were found through histopathology. The mean and standard deviation of the mean cycles (Cq) from blood (Cq = 34.87 +/- 0.60) and nasal swab (Cq = 34.61 +/- 0.87) samples were between 107 and 490 TCID50/ml. Transient infection was demonstrated with a low viral load, but transplacental infection was not possible in gilts. (AU)

Processo FAPESP: 15/08531-0 - Efeito da infecção do vírus da diarreia viral bovina na gestação e em neonatos suínos
Beneficiário:Daniele Araujo Pereira
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 16/21421-2 - Patogenia da infecção pelo vírus da diarréia viral bovina em suínos: aspectos reprodutivos, neonatais e neurológicos
Beneficiário:Luís Guilherme de Oliveira
Modalidade de apoio: Auxílio à Pesquisa - Regular