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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Xenotopic expression of alternative electron transport enzymes in animal mitochondria and their impact in health and disease

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Autor(es):
Camargo, Andre F. [1] ; Chioda, Marina M. [1] ; Rodrigues, Ana P. C. [1] ; Garcia, Geovana S. [1] ; McKinney, Emily A. [1] ; Jacobs, Howard T. [2, 3, 4] ; Oliveira, Marcos T. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil
[2] Univ Tampere, Inst Biosci & Med Technol, FI-33014 Tampere - Finland
[3] Univ Tampere, Tampere Univ Hosp, FI-33014 Tampere - Finland
[4] Univ Helsinki, Inst Biotechnol, FI-00014 Helsinki - Finland
Número total de Afiliações: 4
Tipo de documento: Artigo de Revisão
Fonte: Cell Biology International; v. 42, n. 6, SI, p. 664-669, JUN 2018.
Citações Web of Science: 1
Resumo

The mitochondrial respiratory chain in vertebrates and arthropods is different from that of most other eukaryotes because they lack alternative enzymes that provide electron transfer pathways additional to the oxidative phosphorylation (OXPHOS) system. However, the use of diverse experimental models, such as human cells in culture, Drosophila melanogaster and the mouse, has demonstrated that the transgenic expression of these alternative enzymes can impact positively many phenotypes associated with human mitochondrial and other cellular dysfunction, including those typically presented in complex IV deficiencies, Parkinson's, and Alzheimer's. In addition, these enzymes have recently provided extremely valuable data on how, when, and where reactive oxygen species, considered by many as by-products of OXPHOS, can contribute to animal longevity. It has also been shown that the expression of the alternative enzymes is thermogenic in cultured cells, causes reproductive defects in flies, and enhances the deleterious phenotype of some mitochondrial disease models. Therefore, all the reported beneficial effects must be considered with caution, as these enzymes have been proposed to be deployed in putative gene therapies to treat human diseases. Here, we present a brief review of the scientific data accumulated over the past decade that show the benefits and the risks of introducing alternative branches of the electron transport into mammalian and insect mitochondria, and we provide a perspective on the future of this research field. (AU)

Processo FAPESP: 14/02253-6 - Investigando as alterações metabólicas causadas pela expressão transgênica da oxidase alternativa mitocondrial de Ciona intestinalis em Drosophila melanogaster
Beneficiário:Marcos Túlio de Oliveira
Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
Processo FAPESP: 17/03806-7 - Avaliação do potencial termogênico da oxidase alternativa mitocondrial expressa em Drosophila melanogaster
Beneficiário:Geovana Siqueira Garcia
Modalidade de apoio: Bolsas no Brasil - Mestrado
Processo FAPESP: 17/02813-0 - Proteômica mitocondrial de Drosophila melanogaster expressando a oxidase alternativa sob diferentes condições dietéticas
Beneficiário:Marina Minari Chioda
Modalidade de apoio: Bolsas no Brasil - Mestrado
Processo FAPESP: 15/14547-7 - Efeitos da expressão da AOX em mutantes da helicase mitocondrial de Drosophila melanogaster
Beneficiário:Ana Paula Campos Rodrigues
Modalidade de apoio: Bolsas no Brasil - Mestrado