Carrier flies of multidrug-resistant Escherichia c... - BV FAPESP
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Carrier flies of multidrug-resistant Escherichia coli as potential dissemination agent in dairy farm environment

Texto completo
Autor(es):
Alves, Taila dos Santos [1] ; Batista Lara, Gustavo Henrique [2] ; ParizMaluta, Renato [1] ; Ribeiro, Marcio Garcia [2] ; Leite, Domingos da Silva [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Genet Evolucao Microbiol & Imunol, Postal Address 6109, BR-13083862 Campinas, SP - Brazil
[2] Univ Estadual Paulista UNESP, Fac Med Vet & Zootecnia, Dept Higiene Vet & Saude Publ, Postal Address 560, BR-18618681 Botucatu, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Science of The Total Environment; v. 633, p. 1345-1351, AUG 15 2018.
Citações Web of Science: 6
Resumo

The life cycle of synanthropic flies and their behavior, allows them to serve as mechanical vectors of several pathogens. Given that flies can carry multidrug-resistant (MDR) bacteria, this study aimed to investigate the spread of genes of antimicrobial resistance in Escherichia coli isolated from flies collected in two dairy farms in Brazil. Besides antimicrobial resistance determinants, the presence of virulence genes related to bovine colibacillosis was also assessed. Of 94 flies collected, Musca domestica was the most frequently found in the two farms. We isolated 198 E. coli strains (farm A=135 and farm B=63), and >30% were MDR E. coli. We found an association between bla(TEM) and phenotypical resistance to ampicillin, or chloramphenicol, or tetracycline; and bla(CTX-M) and resistance to cefoperazone. A high frequency (86%) of phylogenetic group B1 among MDR strains and the lack of association between multidrug resistance and virulence factors suggest that antimicrobial resistance possibly is associated with the commensal bacteria. Clonal relatedness of MDR E. coli performed by Pulsed-Field Gel Electrophoresis showed wide genomic diversity. Different flies can carry clones, but with distinct antimicrobial resistance pattern. Sanger sequencing showed that the same class 1 integron arrangement is displayed by apparently unrelated strains, carried by different flies. Our conjugation results indicate class 1 integron transfer associated with tetracycline resistance. We report for the first time, in Brazil, that MDR E. coli is carried by flies in the milking environment. Therefore, flies can act as carriers for MDR strains and contribute to dissemination routes of antimicrobial resistance. (C) 2018 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 15/15425-2 - Disseminação genética da resistência e pesquisa dos fatores de virulência em Escherichia coli isoladas de animais coabitantes em propriedade leiteira
Beneficiário:Domingos da Silva Leite
Modalidade de apoio: Auxílio à Pesquisa - Regular