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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

An immunoproteomic approach revealing peptides from Sporothrix brasiliensis that induce a cellular immune response in subcutaneous sporotrichosis

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Autor(es):
Fogaca de Almeida, Jose Roberto [1] ; Jannuzzi, Grasielle Pereira [1] ; Kaihami, Gilberto Hideo [2] ; Dantas Breda, Leandro Carvalho [3] ; Ferreira, Karen Spadari [4] ; de Almeida, Sandro Rogerio [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Dept Biol Sci, Diadema - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 8, MAR 8 2018.
Citações Web of Science: 8
Resumo

Sporothrix brasiliensis is the most virulent fungus of the Sporothrix complex and is the main species recovered in the sporotrichosis zoonotic hyperendemic area in Rio de Janeiro. A vaccine against S. brasiliensis could improve the current sporotrichosis situation. Here, we show 3 peptides from S. brasiliensis immunogenic proteins that have a higher likelihood for engaging MHC-class II molecules. We investigated the efficiency of the peptides as vaccines for preventing subcutaneous sporotrichosis. In this study, we observed a decrease in lesion diameters in peptide-immunized mice, showing that the peptides could induce a protective immune response against subcutaneous sporotrichosis. ZR8 peptide is from the GP70 protein, the main antigen of the Sporothrix complex, and was the best potential vaccine candidate by increasing CD4(+) T cells and higher levels of IFN-gamma, IL-17A and IL-1 beta characterizing a strong cellular immune response. This immune environment induced a higher number of neutrophils in lesions that are associated with fungus clearance. These results indicated that the ZR8 peptide induces a protective immune response against subcutaneous sporotrichosis and is a vaccine candidate against S. brasiliensis infection. (AU)

Processo FAPESP: 16/04729-3 - Bases celulares da resposta imune na cromoblastomicose e esporotricose: implicações para terapia vacinal
Beneficiário:Sandro Rogerio de Almeida
Modalidade de apoio: Auxílio à Pesquisa - Temático