The effects of increased heme oxygenase-1 on the l... - BV FAPESP
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The effects of increased heme oxygenase-1 on the lymphoproliferative response in dogs with visceral leishmaniasis

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Martins de Almeida, Breno Fernando ; Oliveira Silva, Kathlenn Liezbeth ; Chiku, Vanessa Marim ; Correa Leal, Aline Aparecida ; Venturin, Gabriela Lovizutto ; Narciso, Luis Gustavo ; Cereijido Bersni Fink, Maria Fernanda ; Eugenio, Flavia de Rezende ; Patto dos Santos, Paulo Sergio ; Ciarlini, Paulo Cesar ; Felix de Lima, Valeria Marcal
Número total de Autores: 11
Tipo de documento: Artigo Científico
Fonte: Immunobiology; v. 222, n. 5, p. 693-703, MAY 2017.
Citações Web of Science: 6
Resumo

Canine visceral leishmaniasis (CVL) is known to affect the cellular immunity of infected dogs, through impairing lymphoproliferation and microbicidal mechanisms. This study examined heme oxygenase-1 (HO-1) and its metabolites, oxidative stress and IL-10 levels in CVL and investigated correlations between these parameters. Additionally, the effects of HO-1 inhibition on the lymphoproliferative response and cytokine production in lymph node cells (LNCs) from infected dogs were evaluated. Forty-four dogs, 24 controls and 20 dogs with CVL were selected. Plasma and splenic levels of HO-1, haptoglobin, soluble CD163 receptor, ferritin and IL-10 were determined using capture ELISA. The HO-1 levels and relative gene expression in peripheral blood and bone marrow mononuclear cells were also determined. LNCs proliferation was evaluated with an HO-1 activator and with an HO-1 inhibitor, in the presence of the Leishmania infantum soluble antigen (SAgL), using flow cytometry. HO-1, IL-2, IFN-gamma and IL-10 were also determined in these cultures using capture ELISA. Infected dogs presented oxidative stress and increased HO-1 levels and relative gene expression, with correlation between oxidative stress and HO-1. The substances from heme metabolism and IL-10 were also elevated in the plasma and spleens of infected dogs. IL-10 and HO-1 levels were positively correlated with one another. Inhibition of HO-1 increased LNCs proliferation and decreased IL-10 and IL-2 production in the presence of SAgL. The increased HO-1 metabolism observed in CVL is probably associated with oxidative stress and increased IL-10, which could be one of the mechanisms responsible for inhibition of the lymphoproliferative response in sick dogs. (C) 2016 Elsevier GmbH. All rights reserved. (AU)

Processo FAPESP: 13/06068-6 - O papel da heme oxigenase-1 na leishmaniose visceral canina
Beneficiário:Breno Fernando Martins de Almeida
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/07496-1 - Papel da heme oxigenase 1 na leishmaniose visceral canina
Beneficiário:Valéria Marçal Felix de Lima
Modalidade de apoio: Auxílio à Pesquisa - Regular