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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

N-acetylcysteine protects against star fruit-induced acute kidney injury

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Autor(es):
Massola Shimizu, Maria Heloisa ; Franca Gois, Pedro Henrique ; Volpini, Rildo Aparecido ; Canale, Daniele ; Luchi, Weverton Machado ; Froeder, Leila ; Heilberg, Ita Pfeferman ; Seguro, Antonio Carlos
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: RENAL FAILURE; v. 39, n. 1, p. 193-202, 2017.
Citações Web of Science: 7
Resumo

Background: Star fruit (SF) is a popular fruit, commonly cultivated in many tropical countries, that contains large amount of oxalate. Acute oxalate nephropathy and direct renal tubular damage through release of free radicals are the main mechanisms involved in SF-induced acute kidney injury (AKI). The aim of this study was to evaluate the protective effect of N-acetylcysteine (NAC) on SF-induced nephrotoxicity due to its potent antioxidant effect. Materials and methods: Male Wistar rats received SF juice (4 mL/100 g body weight) by gavage after a 12 h fasting and water deprivation. Fasting and water deprivation continued for 6 h thereafter to warrant juice absorption. Thereafter, animals were allocated to three experimental groups: SF (n = 6): received tap water; SF+NAC (n = 6): received NAC (4.8 g/L) in drinking water for 48 h after gavage; and Sham (n = 6): no interventions. After 48 h, inulin clearance studies were performed to determine glomerular filtration rate. In a second series of experiment, rats were housed in metabolic cages for additional assessments. Results: SF rats showed markedly reduced inulin clearance associated with hyperoxaluria, renal tubular damage, increased oxidative stress and inflammation. NAC treatment ameliorated all these alterations. Under polarized light microscopy, SF rats exhibited intense calcium oxalate birefringence crystals deposition, dilation of renal tubules and tubular epithelial degeneration, which were attenuate by NAC therapy. Conclusions: Our data show that therapeutic NAC attenuates renal dysfunction in a model of acute oxalate nephropathy following SF ingestion by reducing oxidative stress, oxaluria, and inflammation. This might represent a novel indication of NAC for the treatment of SF-induced AKI. (AU)

Processo FAPESP: 15/11933-3 - Efeitos antioxidante e renoprotetor do alopurinol na rabdomiólise induzida por glicerol, estatina, veneno botrópico e leptospirose
Beneficiário:Antonio Carlos Seguro
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/25567-6 - Efeitos do alopurinol na injúria renal aguda induzida por glicerol
Beneficiário:Pedro Henrique França Gois
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado