Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Ionic surfactants-Glossoscolex paulistus hemoglobin interactions: Characterization of species in the solution

Texto completo
Autor(es):
Carvalho, Francisco A. O. ; Alves, Fernanda R. ; Tabak, Marcel
Número total de Autores: 3
Tipo de documento: Artigo Científico
Fonte: International Journal of Biological Macromolecules; v. 92, p. 670-681, NOV 2016.
Citações Web of Science: 1
Resumo

Glossoscolex paulistus hemoglobin (HbGp) is an oligomeric multisubunit protein with molecular mass of 3600 kDa. In the current study, the interaction of sodium dodecyl sulfate (SDS) and cetyl trimethylammonium chloride (CTAC) surfactants with the monomer d and the whole oxy-HbGp, at pH 7.0, was investigated. For pure monomer d solution, SDS promotes the dimerization of subunit d, and the monomeric and dimeric forms have sedimentation coefficient values, s(20,w), around 2.1-2.4 S and 2.9-3.2 S, respectively. Analytical ultracentrifugation (AUC) and isothermal titration calorimetry (ITC) data suggest that up to 26 DS- anions are bound to the monomer. In the presence of CTAC, only the monomeric form is observed in solution for subunit d. For the oxy-HbGp, SDS induces the dissociation into smaller subunits, such as, monomer d, trimer abc, and tetramer abcd, and unfolding without promoting the protein aggregation. On the other hand, lower CTAC concentration promotes protein aggregation, mainly of trimer, while higher concentration induces the unfolding of dissociated species. Our study provides strong evidence that surfactant effects upon the HbGp-subunits are different, and depend on the surfactant: protein concentration ratio and the charges of surfactant headgroups. (C) 2016 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 13/09829-8 - Estudos biofísicos e estruturais da hemoglobina extracelular de Glossoscolex paulistus, e das suas subunidades monômero d e dodecâmero (abcd)3.
Beneficiário:Francisco Adriano de Oliveira Carvalho
Linha de fomento: Bolsas no Brasil - Pós-Doutorado