Copper affects biochemical and physiological respo... - BV FAPESP
Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Copper affects biochemical and physiological responses of Selenastrum gracile (Reinsch)

Texto completo
Autor(es):
Rocha, Giseli S. ; Parrish, Christopher C. ; Lombardi, Ana T. ; Melo, Maria da G. G.
Número total de Autores: 4
Tipo de documento: Artigo Científico
Fonte: ECOTOXICOLOGY; v. 25, n. 8, p. 1468-1477, OCT 2016.
Citações Web of Science: 5
Resumo

Copper is an essential metal for several physiological and metabolic processes, but a narrow range regulate its effect in phytoplankton cells. It can affect the production of biomolecules and be toxic at concentrations slightly above those required, e.g. decreasing photosynthesis and increasing respiration. The aims of this study were to analyse the changes in growth and chlorophyll a synthesis, and in biochemistry (total carbohydrates, proteins, lipids and fatty acids) of the freshwater microalga Selenastrum gracile after exposure to copper. Exponentially growing cells were exposed to 5 concentrations of free copper ions (Cu2+) ranging from 0.7 (control) to 13 x 10(-8) M for up to 120 h. Free Cu2+ ion concentrations were calculated through the chemical equilibrium model MINEQL(+). We observed that copper was responsible for a decrease in cell density and an increase in total protein and lipid production, but no effect on total carbohydrates was detected. The increase in phospholipids and sterols and a decrease in saturated fatty acids under copper exposure suggest a change in conformation of the cell membrane, by decreasing its fluidity. We suggest this serves the cell as a system to avoid the internalization of metal, thereby acting as a detoxifying mechanism. (AU)

Processo FAPESP: 08/02078-9 - Estudos ecofisiológicos e de processos produtivos do Anostraca dulcícola Dendrocephalus brasiliensis Pesta 1921 e suas implicações nas comunidades zooplanctônica e nectônica
Beneficiário:Maria da Graça Gama Melão
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 09/15511-5 - Análise comparativa da composição bioquímica e do valor nutricional de Dendrocephalus brasiliensis, Daphnia laevis e Artemia salina alimentadas com algas cultivadas sob diferentes condições ambientais.
Beneficiário:Giseli Swerts Rocha
Modalidade de apoio: Bolsas no Brasil - Doutorado