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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Prognostic Value of ADAMTS Proteases and Their Substrates in Epithelial Ovarian Cancer

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Autor(es):
Lima, Maira Assis ; dos Santos, Liliane ; Turri, Jose Antonio ; Nonogaki, Suely ; Buim, Marcilei ; Lima, Joema Felipe ; Viana Pinheiro, Joao de Jesus ; Bueno de Toledo Osorio, Cynthia Aparecida ; Soares, Fernando Augusto ; Freitas, Vanessa Morals
Número total de Autores: 10
Tipo de documento: Artigo Científico
Fonte: PATHOBIOLOGY; v. 83, n. 6, p. 316-326, 2016.
Citações Web of Science: 6
Resumo

Background: ADAMTS are metalloproteases with disintegrin and thrombospondin motifs. They are secreted proteases playing a role in biological processes such as inflammation, angiogenesis, and urogenital development. ADAMTS have specific substrates, such as the proteoglycans (PG) versican, aggrecan, and brevican. Despite data indicating a role of ADAMTS in tumor invasion and metastases, effects played by these molecules in cancer progression are still controversial. In ovarian cancer, the importance of ADAMTS gene mutations was recently described and related to chemotherapy outcome. Objective: To analyze protein levels of ADAMTS-1, -4, and -5, and TIMP-3 in human ovarian cancer classified as benign, borderline, or malignant. We also assessed the expression of the ADAMTS substrates aggrecan, brevican, and versican in these neoplasms. Correlations between overall survival and protein expression were performed. Methods: Tumors were classified according to the WHO Classification of Tumors of Female Reproductive Organs. Protein and PG expression was studied by immunohistochemistry. Differences in labeling were analyzed by percent measurements of stained areas. Results: ADAMTS-1, ADAMTS-5, and its tissue inhibitor TIMP-3 are increased in borderline and malignant tumors compared to benign neoplasms. Aggrecan and versican levels were increased in malignant subtypes compared to benign ovarian cancer. Higher ADAMTS-1, TIMP-3, and versican expression was associated with a shorter overall survival. Conclusions: Comparison of protease, TIMP-3, and substrate expression showed that in malignant tumors all ADAMTS and TIMP-3 expression levels were significantly raised compared to the substrates studied. (C) 2016 S. Karger AG, Basel (AU)

Processo FAPESP: 10/07699-1 - Protease ADAMTS-1 influenciando o comportamento e o microambiente do câncer de mama
Beneficiário:Vanessa Morais Freitas
Linha de fomento: Auxílio à Pesquisa - Jovens Pesquisadores
Processo FAPESP: 13/01092-6 - Influência de hormônios esteroidais nos níveis de expressão e localização das ADAMTS-1, 4 e 9 em células derivadas de tumores de ovário
Beneficiário:Maíra de Assis Lima
Linha de fomento: Bolsas no Brasil - Mestrado