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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Differential ontogenetic exposure to obesogenic environment induces hyperproliferative status and nuclear receptors imbalance in the rat prostate at adulthood

Texto completo
Autor(es):
Pytlowanciv, Eloisa Zanin [1] ; Pinto-Fochi, Maria Etelvina [2] ; Reame, Vanessa [1] ; Gobbo, Marina Guimaraes [1] ; Ribeiro, Daniele Lisboa [3] ; Taboga, Sebastiao Roberto [2] ; Goes, Rejane Maira [2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Campinas State Univ UNICAMP, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil
[2] Univ Estadual Paulista UNESP, Inst Biosci Letters & Exact Sci, Dept Biol, Sao Jose Do Rio Preto, SP - Brazil
[3] Univ Fed Uberlandia, Inst Biomed Sci, Histol Sect, Av Engenheiro Dinz 1178, CP 593, BR-38400 Uberlandia, MG - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: PROSTATE; v. 76, n. 7, p. 662-678, MAY 15 2016.
Citações Web of Science: 2
Resumo

BACKGROUNDExperimental data indicate that high-fat diet (HFD) may alter proliferative activity and prostate health. However, the consequences of HFD exposure during different periods of ontogenetic development on prostate histophysiology remain to be elucidated. Herein, we compare the influence of obesogenic environment (OE) due to maternal obesity and HFD at different periods of life on proliferative activity and nuclear receptors frequency in the rat ventral prostate and a possible relationship with metabolic and hormonal alterations. METHODSMale Wistar rats (19 weeks old), treated with balanced chow (Control groupC; 3% high-fat, 3.5Kcal/g), were compared with those exposed to HFD (20% high-fat, 4.9kcal/g) during gestation (Gmaternal obesity), gestation and lactation (GL), from post-weaning to adulthood (WA), from lactation to adulthood (LA) and from gestation to adulthood (GA). After the experimental period, the ventral prostate lobes were removed and analyzed with different methods. RESULTSMetabolic data indicated that G and GL rats became insulin resistant and WA, LA, and GA became insulin resistant and obese. There was a strong inverse correlation between serum testosterone (approximate to 133% lower) and leptin levels (approximate to 467% higher) in WA, LA, and GA groups. Estrogen serum levels increased in GA, and insulin levels increased in all groups, especially in WA (64.8x). OE-groups exhibited prostatic hypertrophy, since prostate weight increased approximate to 40% in G, GL, LA, and GA and 31% in WA. As indicated by immunohistochemistry, all HFD-groups except G exhibited an increase in epithelial cell proliferation (PCNA-positive) and a decrease in frequency of AR- and ER-positive epithelial cells; there was also an increment of ER-positive stromal cells in comparison with control. Cells containing PPAR increased in both epithelium and stroma of all OE groups and those expressing LXR decreased, particularly in groups OE-exposed during gestation (G, GL and GA). CONCLUSIONSOE leads to prostate hypertrophy regardless of the period of development and, except when restricted to gestation, leads to a hyperproliferative status which was correlated to downregulation of AR and LXR and upregulation of ER and PPAR signaling. Prostate 76:662-678, 2016. (c) 2016 Wiley Periodicals, Inc. (AU)

Processo FAPESP: 11/03596-6 - Efeito comparativo da obesidade em diferentes fases do desenvolvimento sobre a histofisiologia da próstata ventral de ratos: possíveis relações com hormônios esteroides e a expressão da enzima 5 alfa-redutase
Beneficiário:Eloísa Zanin Pytlowanciv
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 11/01612-4 - Efeitos da obesidade materna e supernutrição pós-natal sobre o aparelho genital masculino de ratos: avaliação morfofuncional das células de Leydig
Beneficiário:Rejane Maira Góes
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/16071-9 - Efeitos da obesidade materna e supernutrição pós-natal sobre o aparelho genital masculino de ratos: avaliação morfofuncional das células de leydig
Beneficiário:Maria Etelvina Pinto Fochi
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/18011-9 - Repercussões da obesidade materna e/ou pós-natal sobre as células de sertoli e a expressão de fatores parácrinos intra-testiculares
Beneficiário:Vanessa Reame
Linha de fomento: Bolsas no Brasil - Mestrado