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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Clearing and dissecting insects for internal skeletal morphological research with particular reference to bees

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Autor(es):
Diego Sasso Porto ; Gabriel A.R. Melo ; Eduardo A.B. Almeida
Número total de Autores: 3
Tipo de documento: Artigo Científico
Fonte: Revista Brasileira de Entomologia; v. 60, n. 1, p. 109-113, Mar. 2016.
Citações Web of Science: 6
Resumo

ABSTRACT A detailed protocol for chemical clearing of bee specimens is presented. Dry specimens as well as those preserved in liquid media can be cleared using this protocol. The procedure consists of a combined use of alkaline solution (KOH or NaOH) and hydrogen peroxide (H2O2), followed by the boiling of the cleared specimens in 60–70% EtOH. Clearing is particularly useful for internal skeletal morphological research. This procedure allows for efficient study of internal projections of the exoskeleton (e.g., apodemes, furcae, phragmata, tentoria, internal ridges and sulci), but this process makes external features of the integument, as some sutures and sulci, readily available for observation as well. Upon completion of the chemical clearing process the specimens can be stored in glycerin. This procedure was developed and evaluated for the preparation of bees and other Apoidea, but modifications for use with other insect taxa should be straightforward after some experimentation on variations of timing of steps, concentration of solutions, temperatures, and the necessity of a given step. Comments on the long-term storage, morphological examination, and photodocumentation of cleared specimens are also provided. (AU)

Processo FAPESP: 14/10090-0 - Morfologia comparada das estruturas esqueletais internas da cabeça das abelhas corbiculadas (Apidae: Apini)
Beneficiário:Diego Sasso Porto
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
Processo FAPESP: 12/22261-8 - Relações filogenéticas entre abelhas corbiculadas (Hymenoptera: Apidae: Apini)
Beneficiário:Diego Sasso Porto
Linha de fomento: Bolsas no Brasil - Mestrado